Strain culture
Laccase production
* Other inducers were tested
Monokaryon isolation of dikaryotric strains
DNA
isolation
PCR
amplification
DNA
sequencing
ITS1-5.8S-ITS2
Laccase activity assay
Monokaryotic strain culture
P. sanguineus proved to be a better enzyme producer than P. cinnabarinus and P. coccineus.
P. sanguineus G05.10 produced up to 71000 U/L
Best culture medium contained:
Tween 80
2,5-xylidine
Maize bran
Chinese Pycnoporus strains were better laccase producers than the other analysed strains. Using monokaryotic strains through isolation, improves laccase production.
Geographical origin proved to be a stronger determinant of phylogenetic relations, over taxonomical position.
Geographical origin proved to be a stronger determinant of phylogenetic relations, over taxonomical position.
Chinese Pycnoporus strains were better laccase producers than the other analysed strains. Using monokaryotic strains through isolation, improves laccase production.
THANKS!
All data was retrieved from the article
Molecular clustering of Pycnoporus strains from various
geographic origins and isolation of monokaryotic strains for
laccase hyperproduction, by:
Anne Lomascolo, Jean-Luc Cayol , Marjolaine Roche , Lin Guo , Jean-Luc Robert,Eric Record , Laurence Lesage - Meessen , Bernard Ollivier, Jean-Claude Sigoillot and Marcel Asther