BIOSC 1540: L03 (Quality control)

Computational Biology

(BIOSC 1540)

Sep 3, 2024

Lecture 03:
Quality control

Announcements

Assignment 01 is due Thursday at 11:59 pm.
Assignment 02 will be posted on Thursday.
The last module will be Scientific Python instead of Special Interests
- Lectures are optional
- No assigned homework
- Optional assignments for extra credit?
- Please complete the Kaggle intro and Python beforehand if you want to participate
Optional final is Monday, Dec 16 at 10 AM
- If you do worse, I will not count the final

After today, you should be able to

1. Explain the basic concepts and importance of genome assembly.
2. Interpret FASTA and FASTQ file formats and their role in storing sequences.
3. Perform and interpret quality control on reads using FastQC.
4. Identify common quality issues in sequencing data and explain their impacts.
5. Describe the process and importance of sequence trimming and filtering.

Sequencing provides short, overlapping reads of DNA

Genome assembly is the process of combining our sequencing reads into a continuous DNA sequence

Having multiple fragments that contain the same portion of the sequence improves our coverage

Coverage:

High

Low

Assembly terminology

Raw sequences coming from our experiments

Continuous stretches of DNA sequence from overlapping sequencing reads

Connecting contigs in an unknown order

Multiple contigs with estimated gaps

Let's build the original sequence from small fragments

5'- GTACCTAG -3'

GTACC
TACCT
ACCTA
CCTAG

GTACC
TACCT
ACCTA
CCTAG

GTACG
ACCTT

Original sequence:

Fragments

Potential copies?

Errors

with copies

and errors

Hard, right? This is what we ask of computational biologists working in genome assembly

Assembly quality metrics

N50 is the sequence length of the shortest contig at 50% of the total genome length

First, sort all contigs from longest to shortest

L50 number of contigs whose combined length is at least 50%

(Lower is better.)

(Higher is better.)

Then we can annotate our genome

Cleaning our sequencing reads improves our assembly

Garbage in, garbage out

After today, you should be able to

1. Explain the basic concepts and importance of genome assembly.
2. Interpret FASTA and FASTQ file formats and their role in storing sequences.
3. Perform and interpret quality control on reads using FastQC.
4. Identify common quality issues in sequencing data and explain their impacts.
5. Describe the process and importance of sequence trimming and filtering.

Sequences are stored in FASTA files

>BTBSCRYR
tgcaccaaacatgtctaaagctggaaccaaaattactttctttgaagacaaaaactttca
aggccgccactatgacagcgattgcgactgtgcagatttccacatgtacctgagccgctg
caactccatcagagtggaaggaggcacctgggctgtgtatgaaaggcccaattttgctgg
gtacatgtacatcctaccccggggcgag

One line starts with a ">" and a sequence identification code.
- It is optionally followed by a description of the sequence.
One or more lines containing the sequence itself.

>crab_anapl ALPHA CRYSTALLIN B CHAIN (ALPHA(B)-CRYSTALLIN)
MDITIHNPLIRRPLFSWLAPSRIFDQIFGEHLQESELLPASPSLSPFLMR
SPIFRMPSWLETGLSEMRLEKDKFSVNLDVKHFSPEELKVKVLGDMVEIH
GKHEERQDEHGFIAREFNRKYRIPADVDPLTITSSLSLDGVLTVSAPRKQ
SDVPERSIPITREEKPAIAGAQRK

DNA

Protein

However, base calling is not perfect

Normal sequencing by synthesis

Lagging synthesis by failure to remove blocking fluorophore

Leading synthesis by addition of dNTP instead of ddNTP

Clean

Signal cross-talk degrades quality

Noisy

ML models and algorithms compute the probability of error (i.e., quality)

FASTQ files store sequence and quality

@HWI-M01876:76:000000000-AF16W:1:1101:10853:1000 1:N:0:CGTGACAGAT
NTGTACTTCATCCGAAACTCGTGCTCATCTCTGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG
+
#8ABCFGGGFCEDCFGGGGGGGFFCGEFGGGGGGFGGGGGGGGDEFGGGGGGGGGGGGGGGGGFFF
@HWI-M01876:76:000000000-AF16W:1:1101:16471:1000 1:N:0:CGTGAACTTG
NTTCCAGATATTCGATGCATGTGCCGCTCCTGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG
+
y8BCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGFGGGGGGGGGGGGGGGG

@Identifier

Sequence

Per-nucleotide quality

Quality scores measure the probability that a base is called incorrectly

What does "G" or "8" quality mean?

ASCII-encoded probabilities

We need to store millions upon millions of floats (e.g., 0.92829) per nucleotide

ASCII characters require ~1/4 the memory, and we already have to store nucleotides

One million float32 values are about 3.8 MB

Seems small, but one E. coli genome is ~5 million base pairs and we have multiple copies

Hexadecimal characters have an associated int

Dec  Char                           Dec  Char     Dec  Char     Dec  Char
---------                           ---------     ---------     ----------
  0  NUL (null)                      32  SPACE     64  @         96  `
  1  SOH (start of heading)          33  !         65  A         97  a
  2  STX (start of text)             34  "         66  B         98  b
  3  ETX (end of text)               35  #         67  C         99  c
  4  EOT (end of transmission)       36  $         68  D        100  d
  5  ENQ (enquiry)                   37  %         69  E        101  e
  6  ACK (acknowledge)               38  &         70  F        102  f
  7  BEL (bell)                      39  '         71  G        103  g
  8  BS  (backspace)                 40  (         72  H        104  h
  9  TAB (horizontal tab)            41  )         73  I        105  i
 10  LF  (NL line feed, new line)    42  *         74  J        106  j
 11  VT  (vertical tab)              43  +         75  K        107  k
 12  FF  (NP form feed, new page)    44  ,         76  L        108  l
 13  CR  (carriage return)           45  -         77  M        109  m
 14  SO  (shift out)                 46  .         78  N        110  n
 15  SI  (shift in)                  47  /         79  O        111  o
 16  DLE (data link escape)          48  0         80  P        112  p
 17  DC1 (device control 1)          49  1         81  Q        113  q
 18  DC2 (device control 2)          50  2         82  R        114  r
 19  DC3 (device control 3)          51  3         83  S        115  s
 20  DC4 (device control 4)          52  4         84  T        116  t
 21  NAK (negative acknowledge)      53  5         85  U        117  u
 22  SYN (synchronous idle)          54  6         86  V        118  v
 23  ETB (end of trans. block)       55  7         87  W        119  w
 24  CAN (cancel)                    56  8         88  X        120  x
 25  EM  (end of medium)             57  9         89  Y        121  y
 26  SUB (substitute)                58  :         90  Z        122  z
 27  ESC (escape)                    59  ;         91  [        123  {
 28  FS  (file separator)            60  <         92  \        124  |
 29  GS  (group separator)           61  =         93  ]        125  }
 30  RS  (record separator)          62  >         94  ^        126  ~
 31  US  (unit separator)            63  ?         95  _        127  DEL

Phred quality (Q) is the integer associated with the ASCII symbol

P(!) = 10^{-(33-33)/10} = 1.0

Probability that an error occured

P(Q)

10^{-Q/10}

The smallest value 33, because lower hexadecimal cannot be rendered on screen

P(\#) = 10^{-(35-33)/10} \approx 0.63

Sequencing runs store millions of FASTQ entries

@SRR14933407.1/1
TGTAGGTGTTACAATCTTGCCAGAAATCATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGTATGTTGCAATTACCACAAGTTGATTCATTTGTAAATCTTATGACGAGCTTTGTTGAAGAACCAAAGGCGTAGTCTTAGACTAATTTAAGGTTAGTATTTAATTTTAAAGATCGGAAGAGCACAC
+
DDDDDIIIHIIIIIIHIIIIHIIIGIIIIIIIIIIHHHIIIHIIIIIIHIHHIGFIIIIIIHHIIIIIIIIHHIIIIE<DFHHIIIHHIEHCHIIIIIIIHHHHIIHHIIIIFIIIIIIIIIIIIIIIIIIHIIIIIIIIIHIIIIHIIHIIHHIIIIIIIIIIHIIHHIIGIIGIHIHHHHGIIIIIIIIHIHIIIIIIIGIIHHIIIHIHHG@GHGCGHHHHIIIHHIIH?HGCEGH?EHHIHEHIFH@
@SRR14933407.2/1
CTAGAAATATTGATTTATTGCATGTATAATGTTAAAAGTGCCCTTTTATAACGCTTACATATAAAAGCTTATTTAGGGAGAGGGATATTCAACAAGGGGGATTTGAAAATGATAGAACTTAATGCAATTACAACATTATGTTTAGCCTGTATACTTTACTTACTTGGTAAAGCTATCGTTAATCACGTTAATTTTTTAAAACGCATTTGTATACCAGCACCAGTGATTGGTGGCTTAATCTTTGCTATTTT
+
DDDDAIIHIIHIHIHIIIIIIIIIIIIIIIIIIFGHIIIIIIFIIIIIIIIIIIIIIHIGHHIGHIIIEHIIIHIHHIDHHHGIIIIIIIIHIHHHFHHHCEHHIIIIGHCHHHHIIIIIIIIIHIIIIIIIIIHIHIIIIHIHIIIIIIHIHHHIIHIHIIIIIIHIIIIIIIHHHIIHIGHHHIIIIIIIIHIIIHIIIIIHHGIHIIFHHGEHIIHEHIIIIHICHHHHC??HHAGEEHHHEHHIIII
@SRR14933407.3/1
NTTTGTTCTACCAGGAATTGGTGGTTTTTCATGAATATGCTTTGATACTTCTCCAATTCCAACGACAGATTGATTTTTCGTTCGATTATAAAAAATAATATTGTCGCCTTCTTCTAACTGAGTATAAAAATGATAACCATTACGTTTAATACCGTTGTACGTGTGCGTATAAATCGTATATTGGTTTCCAGGTTCAAATTCTTCAGTTTCAGCTAAAAAGAAATAACGTGGTATCTTAATTTCGCCTTTAC
+
#<<DDEEHIIGHICCECHHHHIIIIIIE?GEGCCHIIEFHIIECEEHGHHIHIHHHHCHIEFFEHHHDCHII?HHHHIEHHIHIGHHHIIHFFHD1GHEHGF1FHEHII=HIIHHCH?FHEHHHFHIIGFHGHHHIIIIGHHHHEHHIFEHHIIEF=E?FE@EHHEH0EHHIHEHDH@HHHEE/<CGFCGC?DEHIIHHIIIGC.F@GH?FEHH@FGFFEEFHHHEHHH.GA@CEHIIGHHIGGH-BA7A.
@SRR14933407.4/1
ATGAAAAATTAGCGCAAGCGAAACAAGATGCAATAGCAAATCTAGATACGTTGCGTGACTTAAATCAACCACAACGTGATGCGTTACGTAATCAAATCAATCAAGCACAAGCGTTAGCTACAGTTGAACAAACTAAACAAAATGCACAAAATGTTAATACAGCAATGAGTAACTTGAAACAAGGTATTGCAAATAAAGATACCGTCAAAGCAAGTGAAAACTATCACGATGCTGATGCCGATAAGCAAACA
+
DDBD<<FHE@GHICHIIIGIF<CHIGFEHHHHHH@HHFH?GGHHGHG@HIIH@HDHH0EHEGHFHIIHIGDEGDGHHHHECHHDGCHEDHGHEFHFHHHHHCH@DFEH1F?EEEEHHHHHHHID@HCCFHGHIIIHHHHHHFHE?GH?HGHIHHIICEHEHEEHIHFHHHIIHHHHIHHEEG/C<GE.;DHHHGEC@.C@HHEEBE.ABB.B.BHHEEEG@FHFHEEB7BBEE.6AAGEDE57@GEHH@EC
@SRR14933407.5/1
AAAATAAAACAAATGCTCACATAGTAGATTCATTTTATATCGTTAACTATTAACGTTTAAATTGATATGTCCCTATGTGAGTTTTTATTTTACAAATTTATACTATTTTAATCTCTGACTAAAGTTTTACATCGCTATCAGTATTATGTATGATTTATTTTAACAACTATAAATAAGCTTGAATTTTGTAACTAGTAAGTGTATAACAGTAATGAATACGAATGTTAGATATTCAATAAACTTAAAAGGGG
+
DDDDDHIHIIIIIIIIIIIIIIHHHIIIIIIIIIIHIIIHHIIIIIHIIIIIIIIDHGIHHIGHIIIIGIIIIIIHFHCHIGHIIIIIIIHHIIHIIIIHIGHIIIIIIIIGIIIHIGIIIIIIFIHHHIIIHIIIIIIIIIIIIIIICHHIIIIIHIIIEHHIIIIIGIIGHHHHHHHHEHIIIGHHIIIHGHHIIHHIIIHHHIIHIIGHHHEEHIIFIGIICEF/<GF@GHCHGHIHII?EEHIIIGE
@SRR14933407.6/1
AGATAAATATGATTACTTAACAGGCTTAGGTAATGTAAAAGAATTTTATAGACATTTAAATGAAATTTCACGAAAAGCTGAAAAAGAACATCAAAGTATCGCATTATTATTAATCGATATCGATGGATTTAAAGATGTCAATGATACCTACTCACACAAATCAGGTGATGCTGTATTAAAACAAATGTCTCAATTACTTAAAAACTATGTGCCGAATCAATTTAAAATTTTTAGAAATGGTGGCGAAGAGT
+
DDDDDIIIIIIHIIIIIIIIIIGHHHHIHIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIDHIIIHIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIGHHIIHIIIIIIIIIIIIHIIIIIIIIIIIHHIIIIIHHEGHIIIIIIIHIHIIIIIHIHHIGHIHIEHIIIGIIHHIIIIIIIII.
@SRR14933407.7/1
GGCACTTTATTGTAAGGTGTTTACAACCACGAAAACACATTTAAGTATTGCCAATATGAGCGATAACTATCTTATACTAACTGAGCACGTATAGAGAAAATTATTCTTTAAGACCTATTGCACGTAAATTAGACCGATCCGCATCTACTATCTTACGTGAAATTAGCAGAAATAATGTAAATAAGTTATATCAATCTGAAACGGCTCAAAAGAATTATGTAACTACAAGAAAACTTTGTGGTCATCCTACT
+
DDDDDIIGIIIHIIIIIIIIIIIIIIIIIGIIIIIIIIIIIIIIIIIIIIGIIEHIIHHHIIIIIIIIIIIGIIIIIIHIIIIIIIIIICEHHHIIIIIIIDHIIIIIIIIIIIIIIIIIIIIHIIIIIIIGFHIIHIIIIIIIIIIIIIGIGIIIIIIIIGIIHHHIIIIIIIIIIHHHHHIIIIHHIIIIIIIIHIGGHIIIIGHFHIIHHHIFHHHEHIIIIIIIIIIIHHHIIIHHDHFAFHII?G?
@SRR14933407.8/1
ATTAATAATCATTAACTTTTGCGAACCTTCTTTTAAATAAATAATGCTTCCTATTGTTTCCATTTATTTTCCTCCTTTGTCACCATCTCGATTTCGATATTTCTTCGCATTTCCAAGTCAATCATCTCTTCTTCCGCTTTACGAATGTCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGTT
+
0<DDDHIEE?GEEHHHE1DHDF?HH/CF1C1FFHIH@CHHHGHCDE1D1GCEHHHFH?HGE1E<F<C<<GECFHHIHGHIHHCCE@@CGC1C1EGCCEHHHCHDHHCHDGHFCCH11<<1@<1<G@CGCE?DG@G00EHHH?C1@GCF??G@DCC1<DEH1CHCGH1CH?@G10<CHH0CG?E=0F/<F/CCEH@C-CGHHCC.:-.DEE@..;;C@.9.?,,-7G.;FA..66..8-B5..8.9.BE7.;
@SRR14933407.9/1
TATTAGTAGCAGTGCTTGCGGATTTAGTAACTATGATTTTATTAACGGTCTATGGTGCAATCAATGGTCAAGGCGGCAGTACAATATGGTTAATAGGTATATTAGTTGTTTTCACAGCAATTTCATATATTTTAGGTGTTCAATTTAAAAGAATGTCATTTTTACAAAAATTGATGGATGGTACGACGCAAATCGGTATTCGTGCGGTGTTTGCATTAATAATATTATTAGTAGCCCTAGCAGAGGGAGTT
+
@D?DDHH1GHEHIGEGHE@FHD/C@G?HHIIIIGF<GGHHFHFEHIIIEHHEEEHHIGIHGIICHIIIIIEGHIIGGHIIIIIGHE@GEHHHHHHIIHHGIEHIIHIIIHHIIHGHHHHHHIHIIHIIHHHHIIIIHHIIHHHHIHIGIEEHHHIIIIIIIIIIIIEFEHICGEHEEEFH?GHIGHHDHIIIHIH=HHIIIE@EGC<.EAGA.AHHHIIIEFHHHI?@@BFHHHHIHFHCFEHGECHHC9.
@SRR14933407.10/1
GTAATTTTCTAGCTTCATCCATCGATAATTCAATGACTTTTTTAGCTCTAGGTGTATAATGCAATGTACCAACATGATCTTGACCATGTCCGATTAATTTTTCAACTTCTTCAATTACTTTATCTTCAGTGATATTAAAACTTTCTAATACTTTTGCAGCAATTCCTTCAGGTTCTTTCATTAACCCCAATAATAGGTGTTCTGTTCCTATATTTGAATGATTTAAACGAATTGCTTCTTCTTGTGCATGG
+
DDDBDGEEHGHE@FHCCHH@HCGHCFHI@EH?HHE1<<FEGHII??GHEHEHI@GHHHHEHEHHGEHCHFHHHH@@FHHIF?EEEHIHH1CEH=HHIIIHHG@HHGI?1CG?GHF1<CGFG1CG1FCGHEGEHCHHFEGHF@?GFEEFHFHIHHHH1FG@GFH?C@CEEHIF@G?HHIII1DH?D@G0CGCH<FG?<FFEHCH///<DGHHIGHHI?/</<C/F/FH@/D=GCC.8F.D..C7DDD.:.9.
@SRR14933407.11/1
TAACTGAAGGTATGGTATTAGCTATTGAACCGTTTATCTCATCAAATGCATCATTCGTTACAGAAGGTAAAAATGAATGGGCTTTTGAAACGAGCGATAAAAGTTTTGTTGCTCAAATTGAGCATACAGTTATCGTGACTAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAGAAAGAAGCAATGCCAGAGGAACAAAC
+
DDBDAFHHHIFHFGHGCHIGHIIIIIIIIHHHHIHHIIIIGIGHIHHFIIIIIIHIIIIGIIHFHIIHIIHHHHHIHHHEHIEHIHHHEHHHHIIHHIIIIIIIIGHGHHGHHIIHHHIEHHIIIIIIHIIIIIEHIIIIHHIIGHIIIHHHIIHHHHIIIGHIIHFHHIIIHHHHHIIHHEEHIICFHHHIIIGHHH8CC.:;.::@FHHHHI<EH?E7,--;6.8.;.-.-8-----8-858.8--...
@SRR14933407.12/1
ACCAGCTGCATTAGAGGCAATTACAACCGAGAACGGGTTAATAGTTGAAAAGGTACTACCGACAGAGCTGGCAAGGAATATGGCACCAACAGAAACGATAGAATCGTATCCTAACGCTATAAATATAGGAACTAAAATCGGATAGAATGCGACAGCTTCTTCTTCAATACCACATAACGTCCCGCCGATAATCATTAGTATTGATACAAACACAATTAGCATAAATTCATGCCCTTTAGTTTTCTTTGTTA
+
DB@DD1FG?HHHGIIIIIIHIHIIIIHIIHIIHHIIIHHIHIGHHEHHHEHHIEHIIHI?EDEHIHHIIHHIHHIIGHHHCFHGF@EHIHHFHEHHI=GH@FHHIEHHIIIIIIIIIIIHIHGHHIIHH1DHHIIIIIIIIIIGIIGEHIIIIIHGIIGHGHIIIHIIIIIIIIIHHIIIHIIIIGH<EDEBGH?FHGBHEHICEHC@HHEHHHFHHGIFHH8F6AHHG@GHIIHHHHEHHEEHEHHIGG7
@SRR14933407.13/1
TTTGTCGCCATGGCATTTCTCTTTGATTTTCATCAAACCAGTGTATCAAATTTTCTTTAAAACTAGACTGCTGATACATTTATAAAACCCTTTCCTCACCAAAATTAATTGTCTTTACTCATAATGTTTTTATTGTACATTAAAATCATGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATC
+
@DDD@HEE0EEHHGGHHHEHIGHHG<<<CFHHIE1DGHE@HHHHECFEFFHIIIHEHHHHIIHIIIHIIIIIFCHHIHHHEIIIIIIE=GEHIHHHHHIIIFHHIHFDHEFHHHIIHHEHECH?GHEHHHEEHHHEFHHHGG1GHGFHECGCHCGEHFEHIC1<CFCHEHEEEEGGHH1D?<EHHHHGIHCGHHHHI?FCEHHFGHHHHHHHIIF@F?GH?FEHE//DGGHCC/D?EEE<?CD.7C7CCCD
@SRR14933407.14/1
ATAAAAAATAAATATGATAAAACAGTTTGCGTTAGTAGTCATGTTAAAATACAAACAAATGTAACTCACATTTAATTTGTCATAATGGGAATGTGCGTTTAAAATAGATTTGTTCAAAGGAAAGTGGAGGTGCAATTTTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTGTGGCGCTTGGAATCAAATGGAGGAAATTGTTGAA
+
DDDDDIGIIHHIIIHIIIIIHIIHIIIIIIIIIIHIIIIIIHHFGHIIIIIHIHIIIIIIHIIEHIIIIIIHIHIIIIIIIIIIHIIIHHHIIIIIIIIHHEHHIIIGHHIIIHIIEGHIIHIFHHHHHHHHIIIIIHHICHHHCHHIIIIHHIHIIIIIIIIIIIIIIHHHIIHHHGHIHIIIHIIHIIIIIGHGG//GHHIIEDHHHHFFHHH.?HHHHIIHFHHIIIIHH8@EHHHEHFIHIIIEHHH
@SRR14933407.15/1
GTTCGAATCCCGTCTTCTGCTCCATTATTTTGCCGGGGTGGCGGAACTGGCAGACGCACAGGACTTAAAATCCTGCGGTGAGAGATCACCGTACCGGTTCGATTCCGGTCCTCGGCACCATTTTAGCGCCCGTAGCTCAATTGGACAGAGCGTTTGACTACGGATCAAGAGGTTATGGGTTCGACTCCTATCGGGCGCGCCATTTTTAAATTAATTGAATAACGGGAAGTAACTCAGCTTGGTAGAGCACT
+
D<D@D<FG@CFE1DFEHH?C1FEC1C1<<<F<1CC/?C<</1/C/E/1D1D10<FHHC?HFE@?@FCGHI1FEECEH/D/E?E1@F1FHHHH101<<ECED11<<C1/<CD1<EHHDHGGFEHHIHHIHHHDHDGGCC?FHIHI?00=DF??D@DE?DDHFECEHDFEEG@DBHHECE?::B?E::8BA?8/EDCHH<?H<-../.;B.;FH.AF@@?FHHCGE,.579AF.88-8GAGHID?FHFHA.B9
@SRR14933407.16/1
CTAATAATGATTGTTAAAGTGGTCGTCATACCTATAAATGGCAGTGTTCGATATTTAAACTGAATACCATAAGAAATAATTGCGACACCAACCGGGAACATCCAAGTGACCAACAATGTCGTCTTAATCATATCATCTGATACCGGTAGCAACACATGTACTAACAATCCCGCAACTAATGCTAATCCATAATGTAAACATAAATATTTAATAGTAGCAGGTATATACTTTCTTTCCAGAGTAAAATTCAA
+
D@DBDHHIFGHEEF11DCEFC1<GC/C<GFHHIHIIEGFE?GEHICG1DGCEHGFHHHIHEHHIIHCHCCGHHIIIEHHHIIF=HHIDHHEFHCHI?EHFDDFHHIIHHHIGHIH?GHHEGGHIHIIHIIIIIIIIIIGHIIIIHIIIIIHIIIIGIIIIIGEHHIIIHHFIHIIHIIIICHHHIIIIIHIGHIHHIIIHIIHHEFFAAGCHHHIIICHIFGHIHG?FHHHEHHH@A.BGHIIIIIIECC@
@SRR14933407.17/1
NAAGGCAACATGGTAGAAACTTCTAAAGCCATATTCTTTAGATTATATGAGTTTATGTAAATTATTTAACGATAATAGCAAATTTTCGGCATTTTTTCAATAACTGCTTAGGTAAACTTTTAATAGTTTTATAAATGTACAGAGACGTTTAATGCGAATGAAGACTTATAACTTAATAATGTAAGGGTTTTACTTTATAATAAGAAGGGTAAAAGATTGAATAAACAAATTTTTAAAGGGGCGACAACCAT
+
#<DDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHIIIIIIIIIIIIIIIIIIHIIHHIHIIIIHIIIIIIIIIIIHIIHIIIIIIIIIIIIIIGFHHHIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIFIIHIIIIIIIIIIIIHIGIIIIIIIIIIIIIIIIIIIGIHHHHIIIIIIIHIIIIIHEHIHHHGHIHGHHIHHHHHIHHIHFIGEHIIIHEEDDHIIIIIII@
@SRR14933407.18/1
AATACTGATATCATTAAAAATGATGTTCGTATTCAAAAAGTTATCGATTTATTAAGAGATAACGCAAAGTTCGTTGAAGGAACTAAAGAAGATTAATCTTCATTAAATATTAAATTACAAAAATGAGTAGCAGATGCATAGCTTATGTATCTGCTACTATTCTTTAAGCAAAAAGTTTGTATGTTAATATGTTGCATTGTAATATCCAATCTAGTATAGTCTTTAACGAATAGGGGTGTAAAAAGAATGTT
+
DDDDDIIIHIIHHIIHIIIIHIIIIIHHIHHIGIIHIIIIHHHHIIIIIIIIIIIIIIGHIIHIIIIIIHIIIHHHHHIHIIIIHHHIIIIHHIIIIIIIIDHIHIIIIFIIIIGIIIIIIIIIIIIIIIIIGIHIIIIHIIIIIIIIHHIIIIIFFHIIHHIIIIIGIIHHHHIHHIHIIHIHIIIIIIIIGHEHIHHIIIGHIIIHHHHIIHHHFHHIIGHHIIHIIIEHHHHDEH,DA9B.GEHFHEH
@SRR14933407.19/1
CTCTACTTGCAATCGCATCACTGATGAAATTCAACAACTCTACTTTCTCTTGTTGTAACTTTTCATTGAAATGAATAATAACTGTTCTTTCATCGTCAACAGGACGCTCTTTGTAAATCCATTCTAATTCAACTAAATTATTATACGTTCTAGTACGCTTATACGGTTTAACTTCAACAAATCTGTCCATTTCTTTAAGCGTCATAGAACCTTTTTGCCATAAAGTTAGTAAAATTAAAATTTCTTCTCTA
+
DDDDDIIIIIIIIIIIIIIIIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIHIHHHIHIIHIIIIIHIIHIHIIIHIIIIHIIIIGIIIHIIIIIIIIIIHIIIHIIIIIIHIIIIIIIHHIIIIIIIIIIIIIIHHIIIIIIHEHIIIHHHHIIIIIIIIIDFHIIIIHHHHHIIGHHHHHIIIIIIGHHHHIHIHHIIIIHIIIGIIIIIHEH.
@SRR14933407.20/1
NCAATTTTATGCATTACCAACCCTCCCGATCATGACATTCTTATTCTTCTTTAAATATAGTATACAATGTCACATTTAATTTAAAAAGTTCATATCAAGAAAGTAAATTGGCTGTAATAAAATTTTAATATACGACTTCTTTCTTCACTTATTAAGGCGAAATTTTATCTCAAATCATGTGCGCTATTTCAAATTGAATAATGCCACTGTCTCAACATGTGTTGTTTGTGGAAACATATCTACCGGTGTTA
+
#<<DDHHIHIHIIIGHIIIIIIIIIIIGHIIHIHIIIIIIIIIIGGIIIGHHIHIIIIIHIIIIIIGHHHHIIIHIIIGHFHIIIHHGFHEHIIIIIIIIIF@HHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHGHIIIHEHCGHIHEHHHIIHIIIEHHHIHEHHHEHIIHIIIIHIIIHHFFHHIIHIIHHHCHIIICHHHHHH?HH@GHHEGHFHHFHIH?GHIIIHIG5?EH.
@SRR14933407.21/1
NAACTTTGATAAAGATGTATATAGAAATAACACTGGATCACTGATTGAAACATATCAAATATTTTTAAACAAATTGGAGGATTTAAAATAATGAAAACAATTGAACTACATATCACATTACAACCACAAGTATTAGATACGCAAGGACAAACGCTTACTCGAGCTGTACATGATTTAGGTTATGCACAAGTGAATGATATTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCT
+
#<<@DHIIGHIIIHHIHHHHGIIIHHHHHHHIHIIIIIIIIIIGIIIIIIIIIIIIIIHIHHIIIIIIIIIIHHHIIIIIHHIIFHHIIIIHIIIHIIIIIHHIIIIHIIIIGHHIIIIIIIHIIIIIIIGHIIIIIIIIIIIHHIIIIIGIIIHHIIIIHIIGHIHIIIIIHCEHHHEHHHIIIIIIIIIIHIIIIIIHHIGHHIIIIIIIHDEHIIHIHIIHIIIIHHHIEHIHE@GHDHDHHHEH7@.
@SRR14933407.22/1
TATCAGGATCAAAAGCACCTTCTTGAATAGCAGCTGCTAACCCATATGATTTAAATGTTGATCCAGGCTCATATGTGTTTTGATATAGATCATTTGCCCACTTTTTACCAAAATCTTTACCAGTTTCAGGATTAAATGTTGGTCGCTGACTGTATGCTAAAATTTCTCCAGTTTTGGCATCCATGACAACCGCAAATAAATCTTTCGGCTGGTATCTTTCAACCATGCCATCTAAAGCCTCTTCAACAAAT
+
ABDDDIE?DHHIIIHHIHEHHIHIHGIIFCHCHHIHEHGHHHIHIIHIIIIIIIIIHHE@FHEGHHGFGHFHHHHIIHHIHIHFHGIIHIIGHIIHFHHIIFHEHHIHIHFHHHIHIHECHCHHIIIIIIGHIIHIIHHC?HEHIIIIIIIIIGHIIHIIHIICCHHHIHGHIHIHHIHH0D0@@CF?HHGHIIDHFH<CCGFEHHI-C/-DFHCGFEGHEIHCCHEHHFG@.BFHEEEC..8BGHH?GH.
@SRR14933407.23/1
TCAATCAAAAAGGGGAAATTGCAGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAATCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACATCTGTGTTAGCTGTAATGACGGAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAACCAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATTGTTTGGCAA
+
DDDDDIIIIIHIIIIIHIIIIGIIIIIIIIIIIIIIIIIIIHIIHGIIIIIIIIIIIIIIIIIIIIIF?GHIIGHHHIIIHIIIIIIIIHIIEHIIIIIIIFHHIIIIIIIIHIIIIHIIIIIIIIIIIIIIHIIIIIHIIIIIIIIIIIIIIIGIIHIIIIIIIIIIIHIIHHHIIIIIIIGIHIIHHIIHHIIIIIIIIIIIIIIIIIGIIIIGHIIHIIIIIIIEHIIIIGIHIII<HHIIIIIDHHH
@SRR14933407.24/1
NCAAATTTATCAGGTGCAAATGACGTAGTAGCACATGAGTTAACACATGGCGTGACACAAGAAACGGCGAATTTAGAGTATAAAGATCAATCTGGTGCGTTAAATGAAAGCTTTTCAGATGTTTTTGGATACTTTGTAGATGATGAGGATTTCTTGATGGGTGAAGATGTTTACACACCAGGAAAAGAGGGAGATGCTTTACGAAGCATGTCAAACCCAGAACAATTTGGTCAACCATCTCATATGAAAGA
+
#<DDDIIIIIHIIGIIIIIIIIIIIIIIIIII<<<DFHHGHGHHIIIIIIIIIHIIIIIIIIIIIIIIIIIIHHIGIHHGHIIIIIIIIIIIIIHIIIIIICGHHIHIIIIIIIIHIIIIIHHEHHIIHIIIIHGHIIIIIIIIIIIIHIIIIIIIHIHIIG=HHHIIHHHHIHIGHIHHIHEHHIIIHIGHIIIHIHGHHHEIIIIHHIHIGGHHGHHHHIIIIIIGIGHIHIHAEHGHIIIIFHHHICB
@SRR14933407.25/1
TAAATAACTGAAGCATGTTCGGTTTTAAAATGAGATTCAGCAATAATTTTCAATGTTTCTAATTTATTTCTTGCATCACCGTATGTGGTACTTTCTGATAGAACACCTTCTAAAATTTGTTGAACTCGATAATCTAAAAGTTTTAAGTCTTTATTGATGCATTGTTCGACACACTCTTCTTTGGTTAATGTGATTTGTTCCATAGTGTTCTCCTATTAAGATGTTTGTTTTTCTCCTAAAAACTTATTAAC
+
DDDDDIIHHIHIIIIHIIIIIICHCHGHIIIIIH@@HHIIIIIGIHIIHIHHHIHHIIIIGIHHIIGIHIIIIIHIIHIIFHIIIIIIIIIIIHIIIIIIIGHIHHIIIIIIIIIIIIIIIEGHEHIHEHIIIGHHGHHIIHHIIIGHIIIEHHIIGHEHIHHIIIHHHHHHI=1FHHHGIEEHHIIGFHH0ECHHHHGHEEHHEFHHH@?HHEEHHHFH@FHH/FHGHIIIEHHEHHID.7DHFHHGIE?
@SRR14933407.26/1
GAAATATTCGTCCAAATGTCGTTGTAGAAACAGATCGATTCGAATCAGCAGTTGGATTTGTTCATCTTGGCTTAGGTTACGCTATCATTCCGAGATTTTATTACCAATCATTTCACACGTCCAATTTAGAATATAAAAAAATTCGTCCAAACTTATGCCGAAAAATTTATATCAATTATCATAAAAAGCGTAAACACTCCGAACAAGTACATACATTCGTACAACAATGCCAGATCGGAAGAGCACACGTC
+
DDDDDHIIHHHHIIIHHIIIHIIIIHIFHHHHIIIIIHIIIIIHHGHHIHHIGHGEHGHIHIIIIIIGHHHIHIIHIIIIHIIHHIIGHHHHHGHIHIIIIDGHFHIHHHHHHHIIGIIIIIIIIIIIEHIIIIHHHHIIHIIIEGHHIIIIHIIHCEHHIHHHHEHHIHIIHIHIEHHHHIHEHHIHHIIIIHIIIIGHHIGIIHHHIIIGHEHGHGIIFEHHIGHIEGHHH@CHHHDHEEEHE@GHDH?
@SRR14933407.27/1
TAATTGTGTAGAAATATGAATTTCACTAAATGTTAATAACTTTGTGACGTTTTAGTTAACAGACTAATAAAAATTTGAAAATACTATATATAGTGGTATAACGTAATGAGTAGACACAATATATAGGAAGAAGGGGTAAAATGAATCAAATCGAAGAAGCATTAACGGGTTTGATTTCTAAAGATCCTGCTATTGTTAACGAAAATGCCAACAAAGATAGTGATACATTTTCAACAATGAGAGATTTAACA
+
DDDCDHHFHIHHIFHIIIIIIIIHHHHGGHHIIIIHHIFHIEHIEGHHHGHIEHHDHHHHGHHIHIHFHHIIIIEF@EHHHHHHIIIGHHICEHHHHHHIIGHHHHIIIIFHCHFHHHEHIHHHIGIIHHHHFGCECHEHHFHHEEHIIIGIIIGHHHIIHIIHHHICEHEHHC?@@HHHIHCGHHIH@HIIHH0DEGFH=EHH.GEHCEEHH/D@E/FHCGHEHHFHHEHIHIHEHHGE?GHHIHHGIIH
@SRR14933407.28/1
NACGTAGTATTGCGTTACAATTAGCAGAAGAAGGATATAATGTAGCAGTAAACTATGCAGGCAGCAAAGAGAAAGCTGAAGCAGTAGTCGAAGAAATCAAAGCTAAAGGTGTTGACAGTTTTGCGATTCAAGCAAATGTTGCCGATGCTGATGAAGTTAAAGCAATGATTAAAGAAGTAGTTAGCCAATTTGGTTCTTTAGATGTTTTAGTAAATAATGCAGGTATTACTCGCGATAATTTATTAATGCGT
+
#<DDDHHIIIIIIIHIIIIIIIHIIIHIIIIIIIIHIIIIIIHIIIIIIIIIIGHIIIHHIIIIHIHIIIIIIIIIHHIIHIII1FHHIIIIIIIIIIIIHFHHHHHIIHHIFIHIIIIIHIIGIIIIIIIIIIIHHIIIIIIIIIIIIIIIIIIIIGHIIIIIIIIIIIIIIIIIHCHHIEHIIIHIIGIIIHHIIIIIIICHIIIIIHGHHHHIEHHIIIIIIIHCHCHHI<?EHIIIFGHGHGEHIHB
@SRR14933407.29/1
TCCAATTACAACCTGGTGATATTATTATTACAAAAGGCCCTGTCATGTGGGGATTTTTTGGTCATTGTAGTATCGCGATTGATGATAAAACGATTTTGCAAATTGAGGGGCCTGGCGACAAACCAACTACACAATCCTTCGAATCTTTTAAATATAATTATGCAAGTGGCAAAAATGATTGGATGAAAGTTTATCGTTGCAGCTATCCTGGTGCAGGTAAAAAAGCGGCTGACTGGGTTAAGAAAAACTAT
+
ADDCBHIIIGIIIHHIHIGEHIIIHIIIIIHIIIIIIIHHIIIIIIIHHICH@EHIIICHIHHIHEHIIIIHHIIHICHIIHIHIHIGHIIIIFFHHIIIE1CGHCHHHIIIIIIIHIIIIIIIIIHHIIHHIEHIIIIIIIIIIIIGIIIHGGHIIIIIIEHHHIIIIII?HHIIIIIIHHHHIFGHHDGHIIIIIHCHHIIGHHGHFH?HGH?HHHHHIIIIHIHC<ECEHCGHGAGHEHGIIHEHEH.
@SRR14933407.30/1
ACAATTTTTTGTGTGAAGAGGAAGGAGTCTGTACCATGAATACGATAAGAAATAGTATTTGTTTAACGATAATAACTATGGTATTGTGTGGATTTTTATTCCCGTTAGCTATCACGTTAATTGGTCAAATATTTTTATCAACAAGCAAATGGTAGTTTAATTACGTATGATAATCGTATAGTTGGATCAAAATTGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGC
+
DDDCDIIIHIIEFGHIIHHHHHIHGHHHIIIIIHIIIHIIIIIHIEGHIIIIIIIIIGEHIIHIIIIIIHHIIIIIHGEHHHHIIIHEFHHHHIHHIIIIHHHIHHEEHIIIIIII1FHHIIIIHHIIHGIIHIHIIHHIHHIHHEHIIHIHIHIIIIIHHIHIIFIFHHIHHGHHIHIGHII0FCHEHEFGHEHEF@GHHIFHHIGHHHHHIIIIIIHHIGHCGHHH@@BFHHHEHIHICCEHEEHHH@F
@SRR14933407.31/1
TTTTCATCTTATACTATGTGATTTTCCGTGTAGTAATCCAAGTATTTAACTTGAATACGATTGGTAGAGGTGAAAATGAATTAGTTGATCCAACAGTTGTAAAAGATAATATTGCTCCTGGTGAAAATGATATTAAACAAAGTAAATATCATAATCATGCTATACAAATATTAGAAGGTTTAGGTGGTCAAGAGAATATTGTTAATTTAACCAATTGTGCAACAAGGTTACGTCTAGAGTTAAAAGACACA
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIEHHIHHIIIIHHIIIIIIIIIHIHIIHIIIIIIIIIIIGHIFHHIHHIIIHHIHIIIIIIIIIIHIIIIIIIIIIIGHHIHHGHIHHIIHHHHHHEEHFHHH?HHIHHHHHHHHIEHHHHIII?HHHGHHHHIHIGEHH?FIGI?GHHIHIHHFHHHHEHHIIIIIGHIE@CC@/D@FGHIHEHHHCFHH.
@SRR14933407.32/1
TGCATTAGAACATTTAGATAATCAGCAACTACACGAATATTTAGCTCAATTTGACGAGGCTTCTGCCAAAAATATTCATCCAAACAATCGACAGAGAGTATTGCGCGCTATCGAATACTATTTTAAAACAAAAAAACTTTTAAGTAATCGTAAGAAAGTGCAACAATTTACTGAAAATTATGATACATTATTAATAGGGATTGAAATGTCGCGTAAAACATTATATTCAAGAATAAATAAACGTGTTGATA
+
DDDDDIIIIIIIIIIIIIIIHIIIIIIIGIIIIIIHIIIIIIIIHIHIIIIHIIIHIIIIIIIIIIIIHHIIIIIIIIIIIIIIIHIHIIHIIIIIIIIIIHHIIGIIIIIIIIHHIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIHIIIIIIIIIHIIIIIIIIIIIHIIIIIIIIIHIIIIIIGHHIIIIHIIIICHIIIIGIIHIEHIIIIIHHIHIHIIIIIIIIGGAGHIIIIIIIHHHHHIIHI.
@SRR14933407.33/1
CTGCTTTAGCAATGAGTATGATAAAGTTTGGCATGGATTCTTATGGACACTCACAATTACCGAGTGATGGCTTAAAACGTTTAAATCGTGTTGTTGAAAAGAATATTAATCAAAATATGTTCGTCACAATGTTTTATGGTTTATATGAAGAAATGAACCATTTATTATATTGTAGTTCAGCTGGTCATGAGCCTGGATATATTTATCGCGCTGAAAAAGAAGAGTTTGAAGAAATTTCAGTTAGAGGTAGA
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIHIIIIIIIIIIHIIIIIIHGHIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIHIIIHIIIIIIIIIIHIHIIHHIHHIIIHIIIIIIIIIIIIIIIIIIIGIHIIIIIIIIIIIIGHIIIIIIIIGHHIHIHGHIIIIIIIIGHHIIIIIIIFHHEHIIHIHIHIIHHH?
@SRR14933407.34/1
TGCTATAATTTTGAATTAGAAGTAATTGCGAAAAATGAAAATAACGATGTCGTTGGACACGTTTTATTAATTGAAGTAGAAATGAATAGTGATGATAAGACGTATTATGGTTTGGCGATTGCTTCTTTATCAGTCAACCCTGAATTGCGTGGACAAAAATTAGGTCGTGGCTTGGTTCAAGCAGTAGAAGAGCGTGCAAAAGCGCAAGAGTATAGTACGGTTGTTGTAGACCATTGTTTTGACTACTTTGA
+
DDDDDIIIIIIIIIIIIHIIIIHIIIIIIIIGIIIIIIIIIIIIIIIIIIIEHIIIIHIIHH??EHIIIIIIIHHHHIIGIHIIIIIIIHICHHHIIEHHH<DHIIIIIIIIIHIIIEHGIHHIIIIGIIIIIIHHHGHHIIIIIIIHHIIIIHHFHIIIIIGGHIHIIIHHIIIHHHIIIIHIIIIIHIIIIHIEHIHIEHIIIIGHCHHHHHHHHHIIIDHIIIIGHHHHG?HHF.GH?EHHHCFHII.
@SRR14933407.35/1
TACACCACCAGGCATTAAAGTTTCAGCAACCTTCATTGCTTCTTCTGACTTCGTATATCTCATAAAGTAAATTCCTCCTATAGTTTATGGAAAATCATACATATAAAACCTTATTTATCTAAATAGCGACAAATGTCCTTTGCAAAATACGTAATAATCATATCAGCACCTGCACGTTTCATTGAAACCATTTGTTCCATAACGACACGTTCTTCATCAATCCAACCATTTTGTGCCGCTGCTTTAGTCAT
+
DDDDDIIIIIGIIIIIIIIHIIIHIIIIIIIIIIGIIIIIIIIIHIIHIIIIIIIIIIIIIIIIHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHHIHIIIIFHIIHHIIIIIIIIIIIIIIIIIIHHHIIIIIGIHHIIIIHIHHIIIIIIIIIHIGHHFHHIIIHHIIIIIIIFHHIHIHIIIIIIIIIIIIIIIIIHIHFHHHHIHHHGEHHEHHIIIHCHHIIIIIHIIIHHIHII,6CHIHGCHIGE
@SRR14933407.36/1
GACTAAGAATAATTGTTCTTGAAGTCTTTTCTTTAAATGATGTTCATTATATGAAGCTTCTAACAAGTGATTAACTGTTGTCGCAGCGTATATATTTAAGTATGTATTAAACCAAGCTTTAGTTGCGACATCTCTAATTTGAACAACATCTTTTTCAGTTGCTTGTCTTACCTTGAACATGACTTTCTCCCCTTATTAACAAGTTTTAATAACGGCATTATACCACAACTTGCTCAATACTTAATAAACAA
+
BADDCIIIIIHIIIIIIIIIHIIIIIIHIIHIIIIIGICHHHHHHIIIIIIIIIIHIGEHIHIIIIIIIHIIIIIIIIIIIIHGIIIIIIIIIIGIIIIII<FHHIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIGIIIIIIIIIIIIIIIHHIIIGIIGIIIIIIIIIIIIHHIHIHHHHHIIFH=GHHIHIIIGGHFHHHHIIIIIIDHDHIHHII@HHHHHIHCEGHIIGHHIHIHHHHHIIH
@SRR14933407.37/1
AAAATTGGGAAGATAGTTGTACGACTATCTATGAATAATTCTCTGTTTTCAGGTGGGAAAACTGTTAATACATTGTCGATATCAATATTTGATTCTGTTTGTTTAACTTCCATTAATCGTTCTGTATATTCACTTGGAATATGTCTTTCTTCCAACATTTGAATAATTCTTTGACTTTTTAATAATTCATTTAGACTAGATCCTAAAATTTTACCTCGACGCGATACAATAAATACATTTGTTACAGTTAC
+
DDDA?FHDEGHGHHHHIIFGCCFHIHHIHEF@<CGHHHHIHHHHH<DE@FC?1<<<?HHHIHEEHCH11CG@HHECHHHICCHHEHHIHE@1FCH@GHHH@DFGEHEHC@HIIEH@1FEGF11DHCCHHIGCEHHH?<@GHHE<<11@GHIEECHHHHHHC@<1C1<<@CEHCHCH1<FHHEHHICEE?<F@CG0<<0<0<FE0DGHE@/CFHFHHGC/EHC<-,.79;CHE.BGE66AGAHHEE..9B79
@SRR14933407.38/1
CCTATGAAGAACTTGTAAGTTTAATTTTAAAATTAAAAGATATTATTTGCTAGTAGGAATTCTGTATAATTAGTATATGTTAACAGTTAAATAAGTTATAATAGAGGAGTGTAGCACTTTGCTATATTCCTTTTTTATTTTTAGGAAGGGTGGGCTTTTATGAAAGATTTGACAATGTTATTACACGAATTAAAAGATATGTCCTTTTTTAATAAAGGAGATATATGTTTAATTGGATGTTCAACATCGGA
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHFIIFHIIIIIIHIHIIHIHIIIIIHIHIHIIIFHIIHIIIHIIIIIIIIHHIHHIIIIIIIIIGHIIIHIIIIIIIIIIHHIIICHIIIIIIIIIIIHIIIIIIIHHIIEFEDGHHHHIGHIGIIIIIIIIIIHHIGGEHIIIIIIHIIIIIIIIIIIIIIIIIIICEHH?EHIIIG@GEHHGEG/<?.DGC..GHEHH..DDGEHHFGHFDA
@SRR14933407.39/1
TTTAACAACGGATCAATCGCGTAATACCGACCATCAATTTATCAACACACAAATTGCGACACTCTTTTTAATCGATATCGTGAGCTATCATTTATTAGAAAATACGAATCTGAGTCAAACTTATCAACATACTAAATCTATTATCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAAGCAACAACCGTAGATATAG
+
DDDDDIIHIIIGHIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIHIIIHIIIIFHEHHDHIIIIIGIHHIIHHHIIIIIIIIIIHHHIGIIIIIHIIHHIIIIIHIIHHGHIIIIGHIIGIHHIIIIIGEHHIIIIHIIHHIIIIIIIHHIIGIGHHHIIIIIIIIIIIHIIIIHHGIIIIGHEHH<DDHHHCGHIGHIIEHECBE?HHIGCEFEEGHGHEE?HH776.B.-.-----8885,5-.---..;
@SRR14933407.40/1
GTCATCTTCTTAGGTATCACAGTTTGTGCTTTGTCATTATTTTCGTCATTTTCAACAGTAGCACTAATTAAATCATGTATTTTTTTATTAATTGTTTTAGGTGTAATACCATGTTTTTCATTATGTTTCATCTGTATTTCTCGACGACGTTGTGTCTCATCAATTGCATACTTCATCGAATCAGTCATTTTATCGGCATACATAATGACTTCACCTTTATCGTTACGCGCAGCTCTACCTATTGTTTGAAT
+
DDDDDHCHIHGHHIIHHHIIIHIHHHHIHHEHHHIHIIIIIIIHDEHIIHIHHEEHHHGHHHFHHE@GHIII?CGHHHHHH@DHHGHHH<DHEHHHH?G?CGEHHIIIHHIIIHHHHHIIHHHIIEE?GEGHI?GFIIHIHHIGHHIHHEECHIHIGIHGHEHHHIIIHFGHHHIIHHHEHHEHHIHGGHHHHE?CEHHHGHIHGH?ECG?HH?HFEHHFE.CFCG@G--EHIIIIHIG.8GH.;BF.B.B
@SRR14933407.41/1
CCATCTAATTCAGTAATTACATCACCTTTTTTCAGACCAGATTGATCTGCTAAACCATTGTTGTCAACTTGATCTACAACAACACCGTTCTTAACTTTTCCTAGCAATTTAACTGCTTGTCTTTCAAAACTATTTAGACTGGCAATATTCTTCATTTTAACACCTACATCGGGATAGTCAATTTTACCTTTTGTTTCTAATTCTTTTACAATCTTTTGTACTTCATTAACAGGTATTGCAAATGACATATT
+
D?@DDHIIHIIHIHFHHHIHHHHIIIIIHHHIEF@GEHII?GGFFHHGGHIEH?HHIEEHIHIIIHIIIIIHIIIIIIIIHEHIIHIHHHIHIIIHH?HHHGHHHHIIHHIECHHIHEHIHIIIGIICHHHHHIGHIHHHIGHCFHGHHHIIIIGHHIIIG?GHFFHGEHGDC/CGHDGHFEHHHHIIHHHEHHIGIIGIHHEHIHHIFHHEHIHHHFEHIIIHIEGFHHHHHHGF?.DC:FG..8.AGGH
@SRR14933407.42/1
ATTACCTCATATACTGGCCACATAAAAGGTTCTGCCTCCATGTATCGAGTACCAAACTCTAAGAAACCACTATAAGCTGCATGCGATGTGATAGTGTATTGCAAATCGCCAGTTTTTTTATATCTGATATTGCGTGATAAATTACCAGTCCAATAACCCTTATTCATTACTTCTCTAGCTTTCAATTTAGCTCGTACTACATATTCTTTGGCGTTTTCCTGTAAAATATCATCTACATCATCATCAATGTT
+
0DDBBGFEGHHHE1FH?H?<CHEHH?GGGHC@G<F@11<1<<111DGCGHEC@11<<<CF1CEH?CCHHH1F@C<DEFFG?CHE<0<<DFHIH@1<DF1<F<<1<<10<D@E@1F1</<FHCHFFCCFHHHGH=<0<<FDGEE<<11D<GCF@C@CH1<1<F1<0000D0D@<DF0<FG<0</C<C/<CG///<</<F//FCEHC</</D.CE.:-.CF?@HE....B97B.:@8.8B@?.8..;A.6.B9
@SRR14933407.43/1
CCCGAGCAATGCACCTCTTAAACAACATTATACACGAAAGGAGCATAAACAAATGAACACACTATAAAAAACAACCTTCCTCATCACAATGGCAGTTGCGACTTGGAAGGTTTGGAAGATTGAGAAAAACACAAGATTTAAACTTAGAAATTTTGATTATCCAAAAATTAATAATGCTCAGAGCAAATCATTGTTGGATATTGCTAGTAACGATTTAAAAGATATTTAACTGTATTCAAAATTTTCATATC
+
DAADDHCHHHIFHFHHGIIIHCHIIIIGIIGIHHHHCC0/DCGECHIIHHIHCHH?C@CGHH1<CG1DGE/CC@H=FHC@E1DC1DHH1FCHFI@1<<1/<<<EC<1<C@<C<EEHH@E?GHHFE@GHE=C<GHH@HHGECHHHHEG<CEHHH0CGHHHH?FHFDF<GEHHHIHFHFEH/G/GH?HHHHHFGH@C?C..:.CFEEHHH.:A987.;AE@H.-:BAHFEHHHHIHIIGHFHEA.AB.FH?7.
@SRR14933407.44/1
AGATCATTTATGGCGTGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAAGGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGACATGAATGTAAAATCTACAATAAAACAAGGTAAGGTTCACACATTTAGCTAATAGATAATCATAATTAAATGTATGCAATAGATTTAATCTGTTAACATAAGCACTTTATATTATGATAAAATAGAGGCAATAACAT
+
@B@DDIFHHHHGIHHC?CHEHIHC1GHHHHGFHH??CHEC@EG11<FHFF1CGC1DGHHHHIEHFIHHIHIIEHHEH?GHHCHHF?GHIIIHHIHGHHIIIIHIIHHIIHIIIIIIHHHIHHHHHHHIFHHHHHHIHHHIIIIIHGIIIH@GGHIIIGHHIIGHHHFH?GEHIIHHHIGIHHHHEEEHE@GC1CHHF?GHIGHFHHHHHEHHIIIFHHEHH@HGHIFH?CEHIIGHH?CCH/<:FHHHIG@
@SRR14933407.45/1
AGCTCTAACTACTGCATCTGAAACCAGGTCAACCGCTTTATCTTGACCTACAACACGTTTATGCAAGATATCACTTAAGTGAAGTAATTTTTCACGTTCTGTTTCAACTAATTTTGAAACTGGTATGCCTGTCCATTGGCTGACAATATCACCAATTTCTTCGTCTGTTACAACTTCACGAATCATTCGATCTGAATCTTCACCTTGCTCATCTTGGAAATTATCCTCTAATTCTCTAAGCTCTTTTTCCA
+
?DADDHCCHIHHHIIIHIGIIGIHIIHIHEEHHIIH?=GHHHHIHFHIGHHHEHHHHHHHHIGIHHIIIHIIHIHIHIIGHHHIIIHHIIIIIIIIHIIIIHIIIIIIIIIIIGHHIIIIIIIIIIIIIIHIIIIIIIIIIIIIHIIIIIIIIIHIHIIIIIIIIIIIIIIHIHHIIHIHIIIIIIHIIIGICHHHFHFHIIIIIIIGHHIGHICEHC@GHHIGHHIHIEHFHHHHIGIIIHHIIHIIHH.
@SRR14933407.46/1
ATAAGAATTCAACTGATTTATCAGTTGTTGAAGCACCTGCTTTTAACATATCTAAGCCGTACTTTTGAATATCAACTTTAGCAATATCTTTTAATTCTTCAGCTGCTTTAACATCTTGTTGTGTACATGTTGGTGATTTGAAAAGTAAGCTATCTGAAATAATTGCTGATAACATTAAACCAGCAATTTCAGGTTTAATTTCAAAGCCACGTTCTCTAAACATTTTGTATAAAATTGTAGCTGTACAACCA
+
DDDDDIIIIIIIIIIIIIFHIHHHHHIFHGIIIIHIIIGHEHIIIIIIIIIIIIIFIHDHIIIIIIIIIIIEHHHIIGFHHIIHHIGHHIIIIHIIGIIHHGHHIIHIIIIIHIIIIHHIHHEHHIIIGHHHFH?HEHHHHIGIIHHHIIGEHIHIIIHIHDGHHHIIIIHICHHIIIHHIIIHHHIFGHG?GHIIHEHFHHEECHH@GH.DEHIIIHHHHIFC</DGG@HHHFHHIHHCHGHEHHHHHHG
@SRR14933407.47/1
ATGACCGCAACGATACCTAATCCCGCTTCCATACTCTCACGAAGCATACCGCTATTCCCTAGTGTTTCTACGAATGTAATTGCTAAAACGATACTCAATACAAGTCCGGCAATTGCACCACCAATCACACTTGCAGTACCCTTCTTATCTTTGACATTACGCGTCATAGTAGTCAATGTCATTACAATTAATAACACTTCTAGTCCTTCACGTAAAAAGATAATCATCACATCGACGAAGCTATAACTATG
+
D@@BBHDHHIIIIIHHHEIHIIIEHHCCGFHHIIHIHHIEHEEHIHIIIHHHDHEHGHHIFGHHHHEEHHIEHIIHIEHGH?HFHHIHIIGHHD?FGHIIICH@FHIIHIHHIIIIIIEHGHHHIIIHGIII1CGHHHI?HHHIHGFHH<FH0FHCHHHGHHIIIHIIIHICGHIGH?8/=C@CC8.:;?FCEHICGHCHHBC.;AFG?.BABH?AAB@G6GHFFECHHHHHI-BD,EC@EHCF?AHGHF?
@SRR14933407.48/1
ATTAATATGCGTAAAATATTTAGTACAAAATTTACTCACTATTTTACCTTGAAACCTATCTGACTTGGTAATAAATTTTACTTGTTCCTTATTAGTAACGATTGTCATTGATTTTATTGATGGATGCTTAAAAAATGTAAATAAATCATATTCTGAAAATCCTGATTGGCCAGGATAGTTATGTAACATAACAATTGAATTCGGTTTACTGTCAAATAACAATTCGGTTGCTTGTTACCCTGGCACAAAAG
+
0DDD?HHIIFIHHDHCHGEH@FHFHEIIIIC@1CGEGGHHHGE<FHIIGIHHGHIIEG@1CE@G<CCHGHHHI11<GFEC@GECC?<<F1DCEGEFHHHHCHCC<1CFHHHE@@?FHC?FHEFH@DCH@<CGHFCC@EH11F?HHHHIIHEHHHC@HHHIHHCFHFHFGEGHE@@GH1D1D?1CECCF?E?EH0FG0FG0G?0CC=EFEC@DGHGH/<</<FG?G//GH?GHE:DC.FH?GH.8FG?.@B.
@SRR14933407.49/1
GTTATCTGATGATTTGTGTTGAATTATTTGTGTATATATTAACCGATTAACGTCTTGTTCTTCTTGAACGTCTTTCTTCTCTATTTCTAATGAAGCTAGGAATATCATCTTCTTTAGTTGTATGTGTTCTTTCACTTACACTATCAGTTGCTTGTGCATTTGATGAATTTGAAGTGAATGATTCATCTTTAGAAGTTGCATTGCTAGAAGTATTTACGCTTGTTCCGAATCCAGTGCTACCAGATTTACGA
+
DDDDAHIIHHHIHHIIHIIHHIIIIIIIGIHH<CHHIIHHHIIIHHHFIHEHHHIIH?GHIHGIG@HHIHIHHIIHHIIEHHHHIIIIIHIHIHHHHEHHG<FHHIH?GCEHIIIHHGHHHHIEHEFEEIFIIIIIIEGEEHHHHHIIFHIHIEHHHHHF11<D@CCHIH@C?EHHHHHHEHIGHGCGHHHDHH?GHGHHHHHEHHHII@HGFGHIIIGHE?<CHECCEHHGHHHHHIIECGHGHIIHHHE
@SRR14933407.50/1
TCTTAATATAAACATCTATTTCAGTGTTAATTTACAACTATCTACTGAAAATTATGTCATCATAATAAATTATGCAATAAGTATTTGATACCTTTACATTGCTATGTATCTCTAAAAATTGCTCCCACACATTTATTTTATACCCAAATATTTAACTATTTATAATTATAGTAAAAAACATTTTAATAGTATATACTTTTCTCATATTTTTTTGATATAAGTTTTAAAAAATAAGTCGATGTACATCAACT
+
DADDDIIIGHHIIIIIIIIIIIIIIIHIIHIIIIIHIIHIIIIHIIIGGHIIIIHHIIGHHIIIIGEHHIIHHGHHIIIHIIHHHIFHIHHIIFFHHIIHIHHFHCHHIIIGHIIIGIHHHHIIIIIIIIHIIIF1DGHIIIIHHHGIIHHIIHIIHIIHIIHEFHIIIHCHFG@HIIIIIIIIIIIHEHIIIIEEHIIIIIIIIEHHIHEHGI///GCHEHHIHGHHEHHHIHEHCC.CFGHIHHHFEHH
@SRR14933407.51/1
GAAAGACGTTTCAGATTATGAAGACATTTCGGATTTATACTTAATCAGCGATGCGTTAGTTACCGACTACTCATCTGTCATGTTCGACTTCGGTGTATTAAAGCGTCCGCAAATTTTCTATGCGTATGACTTAGATAAATATGGCGATGAGCTTAGAGGTTTTTACATGGATTATAAAAAAGAGTTGCCAGGTCCAATTGTTCAAAATCAAACAGCACTCATTGATGCATTAAAACAAATCGATGAGACTG
+
DDDDDIIIHIIIIIHIHIIIIIIIIIIIIIIIEHIHIIHIHIIIIIHHIIIIIIIFFHIIIHIIIIIIIIIIIHHIIIHIIIIIIIIIIHIIIIIIHIIHIFHIIHIHIIIIHIIIIIIIIIIIIIHIHIIIHIIIIIIIHIIIIIEHHFHIFIIIIIIIIIIHIIIHHHGHHIIIIIIIIHIIIIIIIIIIIIIGHHIIIIIIIIGHHIIIIIIIIIIHIIIHIIIIHHIIIEHHIHHFHIIIIIHIIIC
@SRR14933407.52/1
TATTTCATGGTATGGCTGAACATATGGAACGTTACGATAAATTAGCACATGCACTTTCTAAGCATGGCTTCGATGTGATACGTCATAATCATCGAGGACATGGTATTAATATTGATGAATCAACAAGAGGGCATTACGATGATATGAAACGAGTTATCGGTGATGCCTTTGAAGTAGCGCAAACAGTGAGAGGCAATGTTGATAAACCATACATTATAATCGGACATTCAATGGGATCCGTTATAGCTAGA
+
DCBDDIIIIIIIIIIIHIIIIIIHIIIIIIIHIGGIHIHIIIIIHEHIIIIIIIIIIIIIIHIIIIIHIIIHGIIIIIIIIGG1CGHHECHFHIIIIII?F<GHG?EHIGIHHIGHIIIIGFHIII@HIIHHGHHHHIHHHCHIIIHIIIHEHEGIIIIHHIHIHIIHGCHFHIHIIIIHI<EHHHIIIEFHEDHHIIIIIGEHHHHHEEHHHHGHHHHHHHIIIHHFHIGIIIIHHHHIIEHIEEIHC?.
@SRR14933407.53/1
AAGGATTAAGAAGTTTGAATGGTGGTCGCATGGCAAGATTTGGACGTACACCATTACTTGATGCAATGGAGATGGCTAATGAGCATATTATGGTGATTGCCATGATAGAAGATGTTGAAGGGGTTATGGCCATTGACGATATAGCACAAGTCGAAGGTTTAGACATGATAGTCGAAGGTGCCGCAGATTTATCCCAGTCACTTGGCATACCATGGCAAACGCGTGATGATCAAGTAACATCACATGTTCAA
+
DDDDD@FHIIIIHHIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIIIIGIIGIIIGHIIIIIIIIIIIIIIIIIHIIIHIIIIIIIIIIHHIIIIIIIIIIHIIIHHIIIIHHHIIIIIIIIIIIIIHHIIIGHIIIIIIIHIHIIIIIIIIIHIIIHIGIIIIIIIIIIIIIIIIIHHHHIIIIIHHHIIIHHIIIIIIIIIIIIIIIIIIHIIIIIHIIIHIIHIIGIIIHIGHHIIIHHHIEHHHHIH
@SRR14933407.54/1
ATTTGAAGAAGTGCCATCTTAGATTGGATTTCTTTCATTTTTAATAACCTATCTATTGAAGCGCTATTGGAATCAGAATTATCATATTTAACTAATAGATTTAATAGTTTCACTTGAGTCAAAGTGAGATGAAATGAATATTTCAACATATCATTAATGACTTTCAACATTAGTATGTGTGCAGTTAAACGCTTAACTTTAAATTTAGACATCTTTAAAACCTCTCTTAAACCATGCCTATATCTCAAGAT
+
DDDDDGHIIIIIHIIIIIIGIIHIIIHHHIIIIIIIHIIIHIIIGIIIIIIHIIIIIHGHIHIHIIGHHIIIGIIHIIIIIIHIIHHIIIIIIIGIIIIIIFHIHIIIIIIIIIIGHHHHIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIHIHIIIIIIIIIIIIIHHICHIHIIHIIHIIIIIIIGIIIIHHIIIIIIIIHIIIIHIHFGHHHIDHHIIIIIHGGHHIHIGIHIIHIIGIIII.
@SRR14933407.55/1
TGATCGGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTCTTCGGATCGTAAAACTCTGTTATTAGGGAAGAACATATGTGTAAGTAACTGTGCACATCTTGACGGTACCTAATCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTAT
+
DDDDDHHIIIIHIIIIIIHIIIIIIIIIIIIIHEHHIGIIIIGIIHHHICHIIIIHIIIIIIIIHIIHIDHIIHIIIIIICHIIGIHDGFHHFHIIIIHGI?FHHFHHHIIIGHHHGHIIHHEDHIIIHIIHIHIIIIIIIIGHIIIIIIHGEFGHHIIIHDGHIHHIHHII.FGHGIHIGDIEHHIHICHIIIHHHHC@FHIIIDEE.BBHHEHHHHHHGHHIIIHHC.-9BGB?GHCHHIIHEEEB.EG
@SRR14933407.56/1
TTAGTTTCAGGCGGGAATGTTGACTTAACTAGAGTTTCAGGTGTCATTGAACATGGACTGAATATTGCAGATACAAGCAAGGGTGTGGTAGGTTAAAACATTTAATCTGAAAAATGAGGTGTAATTATGTCAAATGGTAAAGAATTACAAAAAAATATAGGTTTCTTCTCAGCGTTTGCTATTGTTATGGGGACAGTTATTGGTTCAGGAGTATTCTTTAAAATATCAAACGTAACAGAAGTAACAGGAAC
+
DDDDDHHIIIIHIIIIIIIIIIHIIIIGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIGIIIIIIIIIIIHHIIIIIIIHIIIIIIIIDGHIIIIIIIIIHIIIIIHHHIIIIHIGHIIIHIIIIIIIIIIIIIIIIIIIIIIIIIHIHHIIIIIIIIHIIIIHIIHIIHGHIIIIII=CHHIFHIIIIIIHIIIIHIIHHIIIIHHIIIIICGHHIHIIIIIIIIIIIIHHHIIII@
@SRR14933407.57/1
ATATGAAAATATTTATTATTATTTTACTACAACTCGCTTCAATTTACTTAAAATAGACAATATTAATTAGATAGTACACACATTTCTTCATAAAAGTGATTTTTCAAAAATATAAATAACACACTCTTATCTTTTTCAAAATCATTTAATGCTATTTTCATTAAAATCAGCTGAAGCACCAAATCTATTCTGATTCAATCAAGAATACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT
+
DDDDDIIIHIIIIIIHHIIIHIIIHHIHIIIHIIGIIIIIIIIHHIIIHCFHGGHIIIIHIIHH?FHIIHHGHIHIIIIIIIIIIIIIHIIIHIHHHHHGHGHHIIHHIIIIIHIIIHHIIIIIIIIHIIIHHIIIIIIIIIIIIIIIIIIHIHHIGHIGHIHIIIIIHIIIIIIGGHEGHHIIHIIGHIHHEIIIIFFHIIIIIIIIHGHHIIHGHHHIIIIIIIIIIIIIHFHIHHE7FHIGHDEHGHB
@SRR14933407.58/1
AGTGCATGATGTAATCCTGTAGGAATTAATAATCTGTTGGCAACACCATATACGAAAGCCCCAAATGATCCTAAACCAACTATAGATTCACCAAATTTTACAATCCATGAATAAAGTAGTGGCCATAAGAATAACAATATGACAACTAAAAATGTACAGTAAAATGCAGTCATAATTGGAACTAAACGTTTACCACTAAAAAATGATAATGCTAATGGTAATTCTGTTTCACTAAACTTATTGTATGCATA
+
DDDDDHIIHIIIIIHIIIIIIIIIIIHIIIIIIIIIIIIIIHCDGHHIIHGIIIIIIIIHHIIIHIIIIIIIIGIIHIIFHHIIIHIIIHHIIIIIIIIIGHHGHIHIIIGHHHHIIIIIIFGHHGIIIIIIIIIIGIIIIHHIIGIHIHIIIHHIIGHHIIHEHHIIIHHIIIIIIIIIIIIGIIIIHIHIHIIIIIIIHIHHHIIIIIHIIFHIHEHHHIEHHHH?HIHIIHHHIEHGHHHIIIIIIIG
@SRR14933407.59/1
ACAACATGGTATTGATGATGAAAATGCAACAAAACAAACTCAAAAATATCGTGATGCAGAGCAAAGTAAGAAAACTGCTTATGATCAAGCTGTAGCTGCTGCGAAAGCAATTTTAAATAAACAAACTGGATCAAATTCAGATAAAGCAGCAGTTGACCGTGCATTACAACAAGTAACAAGTACGAAAGATGCATTGAATGGGGATGCTAAACTGGCAGAAGCGAAAGCGGCAGCTAAACAAAACTTAGGCA
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIGIIIIIIIHIIIIIIIIIIIIHIIIIIIIHIIIIIIIIIIIIIIIIHIIIIIIGIIIIIHIHIIIIIIIIIGHDIIIIIIIHHIIIIIIIIIIIIIIIIIGHIIIIHIGIIIIIIIIIIIHIIIIHIIIIIIIIIHIIIIIIIHIIIIIIIIIFCHIIIIIIIIGHEHEHHIIIHGHHIIIIIIIHHHIIIIHIH?EHI?<HHHHHIIIIIIIIIIHIHI.
@SRR14933407.60/1
AGTGATATTTCTATAGCACCAACAACGTACACGTTAGATGACATTGAACGTGAGCTTGGTCATGATATTAGTTTTAGAGAAAGAAATGGTAAGATAGTCCGCCTATATTTTAATGGGAAATCATCATTTGTAACATGTTATCTGCAAAATGAACAAAAAGACATTGTGGATCGTGCAGAATTTGTAATTAATAGTATGTTAGTACGTAAAGATTTTTATAGTTATACACGCATATTTTCTGAGTATTATGC
+
DDDDDIIIGHIHIIIIIIIIIHIIIIIHHGIIHIIIIHIIHHHHIIIIHIIIHIGIIIIHIIIGHIIGIIHGHIIHIGIIHIHHIHIIIIIIIIIIIIIIIFHHIIIIIIIHIHIHIGIIIIIHIIIHIIIIIIIIIIIIIHIIIIIIIIIIIIIIHHIIGIIIIIIIIIIIIIIIHIIIGIHIIIIHIHHHHHHHCHHIIG<FHHIHIHHIICHCHEIIHIIIIIIIICHHHHHIIEHIIIIIFHHIHHC
@SRR14933407.61/1
GTTTCGCCTTGTTTAAATAGCGTGCCTGAAGGTAACAATTCGATTTCAACAGGTACAATCTCATCTTGTGACAACTTTAATTCTGTTTCATGTTTATGCCAAGGTTGCGCGATTGTGGATTTTTCTTGATCTAATTCACGATGTGATACGCGTAACCAACCAGTAGCTACTTGACCATTTTCAATATGATTAAAATCAGGGAAGTTAACTTCATTACCACGACGATCTAACTTTTTAATACCTGCAAATAA
+
DDDDAHHHHHHEHHIIHIHHHIHHIIHHHIIIHIIIIIHHHGIIIEHHIIIIIIIHIIIIIIIHIIIHIIHFHHIIHHHHHIIHIHIFHHIIIIHIIEH1C<GHFHHHIIGHIGGCHHIIIIIIIIHHGHIIIIIIIIIIHIHIHFHHIIIIHIIIHHIGH=CGH?HHHHIHEHHHIIIHCHHHIIIEHHIIIFFHFHEFHHIIHGHIIFFHHHHHHHHHAEHDHHHHHEHFEHGGEHHIIHI.FHGB@BE
@SRR14933407.62/1
CAATTTAAGACAACTCAAATAGCATTTAGCTAACAAGTCCTGATTCAACTTTGAAACAGGCAATCGACGTATTTGCGGACCAACCGTATGAATTATATACTTTGCTGGCAAATTATATCCACGTGTTATTTTGGCTTTACCTACACCTTCATTGCGCCCTTGTTGTCGAATAATCTCTGCACAATCAAGTCGAACTTGAACACCCGCTTTTGTATGAATAATATTATCAATGCAGTCATGATTAGCTTGCA
+
DDDDCIIIIIIIIIIIIIIIIIHIIIHIIIIIIIIHIIIIIIGHIIIIIIHIIIHHHIHCHHIIIIIIIIHHIIHIIIHIIIIIIIHHHIIIIIIIIGHIIDFHHIHHGIIGIIGHIIHIIIIIIIIIIIIIHHHGHHIHHIIIIIIIIHIHHIIIIIHHHIIICHHGEHHHHIIIII/@HHIIIIIIHFHIIHHIIIICHIIGHHDCGHICHHGECHHHHHHIG7GGHF@EHIIHHBGHEHHGGEGF@G.
@SRR14933407.63/1
AGAAACAATTGATCATCCAGAAGATTTTGTTCCCTATCAAGATACATCTGTAACACAGCAATTAGAGGATTATCGCTCGAACAATAAATATGTTACTAGCTTTCTATTAATGCCAACAGTTATAGTAGTTAATTCAGATTTACAAGGAGATATTAAGATTCAAGGTTATCAAGATTTATTACAACCTATACTTAAAGGTAAAATTGCGTACTCAAATCCAAAGATCGGAAGAGCACACGTCTGAACTCCAG
+
DDDDDIIIIIIIIIIIIIIIHIIIIIIIIIIIIIHIIHIIIIIIIIIIIGIIIIIIIIIHIIIIIIIIIIIIIHIIIIIIIIIIIIIHIIIIIIIIIIIIIGHIIIIIIIIIIIIIIIIHHIGIIIIHHIIIIIIIIIIIIHIIGHHIIIIIIIIIIHHIHIIIIHIIIIIHIHIIIIIHIIHIIIIIIIIIHIIGIIHHEGFHIHIIIGHIIIIIIGGEHHFHIIHIIHDHIGIIIIHGHHHHBFGIIHF
@SRR14933407.64/1
GCTATATATGTTTCAAGAATATGAAAAAGGCGTGTCGTTTCAATACTTAATCGATACATTACAATTAGATATTAATGTCAATACTTTGTATCCTAAGTATAAATATTATAAAACCCATGGTATTGAAACACTACTTACACAAAAACAATACAATCACTATTCAAAGGAACTAAAATTAAAAGTTGTAAATGAATATTTGAATTCAAATCAATCAACCCAAGACATCGCAATCAAATACAATATTCGTAGTT
+
DDDDDIIIIIHIIHIIIIIIIIIIIIIIIIIIIIIIIHIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIHIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIHIIIIIIIIIIGHGHIHHHHIIHHIIIIIHIIIIIIIIIIIIIIHIIIIHIIIHIIIIIIIIIGIIIIIIIIHHHHIIIHHHIIIIIIIIGHHHIIHH
@SRR14933407.65/1
GTTAGCATTGCGATTAAACATATTCAGGATTCTGTGCCAAATGTGACAACCGATGTACGTAAGGATATTCCGCAATCTTTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATTCAAGAAATGAAAGATGATTTGAGTAGTGTTTTACATGAAAATCGAGCGAATGAAGATGTCTATGAACTCGATAAAATGAAAACGATAGCGGTACCATTGAAAAAAGAAGATCT
+
DDADACHHFHGHHHIHHHEHHHIIHIIIHIIIHHHIIGIIIIGIIHIIIGIIDIIHIIHHIHIIIIIIE?FEHHHIIIHIIEHGHHIIHIHHHIHHEECHIEHIIHIHHIICGHIIIEHIIIHEHEEHEGHEHHHHIHEHIIGHIIEHIHIHIHHHHH?FEHGDHIIIIIIIIGHHHF/CGGIGHHIIIIIGIIIIIEHIIHEAHGHIIIICHHHIIIIHHHFIIIIHHHCEEHHIEHIIIIIIIGIHHIA
@SRR14933407.66/1
GGCAGCGCAGAAATAATATTTTTATTTCCTAGAGACATTTTAAGATTGTTAAATAGAATCATTAGTGAATCTTATTTTAAAGATAGTAATGGATTAATCTAAATAAGTGCGGATAATATTAACATAACAACATAATTAAAAGACATAAATGACAATAAAAGGAGTATAGAAATGACTCAAACTGTAAATGTAATAGGTGCTGGTCTTGCCGGTTCAGAAGCGGCAGATCGGAAGAGCACACGTCTGAACTC
+
DDDDDIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIHHIIIIIIIIGHHIIIIIIHIIIIIIIIIIIIHIIIIIIIIIHIIIIIIIIIIIIIIHHIIIIIIIIIIIIIIHIHIGIIIHHHIIIIIHHHIIIIIHIIIIIIIHHIHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIHHGH
@SRR14933407.67/1
TAAGTCGTTTTTAAGTGAGTATATTTCAAGTTAATTAGATCTATAAAAAATTTAGGATGGATTAAATGTATATTAAGGATGTTTAGGATAATTGGATTTAAATTTCTAATAAGTCATGATTATCTATATTTTGAGAGTCAATATCTAAAATACTATCTGAGAGAATTTCATTATTTTGGGATTTAGGGGACATAAATTTTATTGTTTCAACATATAATTCAGTGACAAAGTGTGTTTGTCCGTCTTTATCA
+
0B?DDGHHHE1CG?FEHEG1DGH?CCHH?CFGHHIHIEF1FEHFHHH@HCGHE@<@C1DEEHCEFHHCGHIIGHGHHH1<FHFH@CC@HI?<1FHIHFHHHGFHGEHIHGIIICGGHEECDGHHIHIIEGHIIIGIIC1GHEHHHIHHHIHGFHHIIII1GHICHHHHGHHFH?CGEFCHH1FGHGEH.<CEE?H@CHHIIIHHF?@@C?GEEHHIIGEHIHHHHH@EH?/DFFC@FCFE7ECHEHHHII.
@SRR14933407.68/1
CTTCCTTAGTGACTTCATAGCGTTCTTTTAATGTTTGAATATTTTGTTCGATTTCTTTATCGAATTGTTTATTATTTTTTAATACATGCTTAACAGCGCTTTGTGTTGGAAGCGGTCCACTAGAAATGTTGCTTCGTATAAGACCTTTTACTTTGGCTTCTAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACACACT
+
DDDDDIIIIIIIIIIHHIHIIIHIHIIIIIHIHHHIIHIIGGHIIIIIIIGIIIIIFHHIIIHHIIIIIHHIIIIIIIIIHIIIIIIIIIIIGIHIHHIIIDGHIIIEHHIHHHIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIHIIIIIIIIIIGHHIIHGIIIHIIIIIIIIHHEHIIHIIHHCGHIIIIIIIIIHECHIIHHIE;.BHHIIIHIIIIIEEH.FEH.96BGHHHDEHD6,,,---8...
@SRR14933407.69/1
AGCAAGGTTTTACAATCTTACGCTCTTATGAGCCAGAAATGTATGGCGAAAAGTTAGACTTAGCACTTATGTACAAAGCATTTTAAGTAAATTAAATATTATATTAAACGAAAAGGAGGGGAATAATGACTAACGCATATGTAGATTTAAAATTAGTAGAAGAAAAAGTTTTTAAAGACCCGATACATCGATATATTCATGTTGAAGATCAATTGATATGGGATTTAATTAAAACTAAGGAATTTCAAAGG
+
DDDDDIIHIIIIIIIIHIIIIIIIIIGIHHIIIIHHEHHHIHIIIIIIIIIIIIIIIIIGIIIIIIIIIIIHIIIIIHIIIIIIIHIHIIIIIIIIIIIHIGHIIIIIIIHIIIGHHIIIIEGHIHHIIIIIIIIIIHHHIIIIIIHHIHHHHHIHHHIIGHHGIDGDHHIIIIIHEGHIIIDEHHIGHIHHHHIIIHICHHIGIHEHHHHHHFHHHGHIGHIIHEHHHHIEHHIIIIIHIGHEHHHIIIG
@SRR14933407.70/1
AACATAGTCACCCATTGATATTTCATCTGTGACAAGATGTGTTCGGATACGTTCATCGTAACCTTCATAATGTCCGCATATGAAAACGATGTGGTCAGCCTTGCTTAATTCAACAGCTTTCTGATGTGAAAATGGCTCGCCTTGTGGACACATTAAAATAACGCGTGCTTGTTCTGTGACATCTAAGTCTTCCATCGCATTAAAAACAGGTTCAGGCTTTAACACCATACCTTGTCCGCCACCATACGGAT
+
DDDBDIIIIIIHGIIIHHGHHHIIIIIIIHHGIIHHGHHIIIGHIGIHIIIIIHHHIIIIIIIIIHGIIIIIHIHHIIIIIHHIIHIIIIIEHIHIGHEHIHHHIIIIHIIIIIHHIHHIIIIGIIHHIHIGHHHIIIHHIHGHIEHHHIIGHCHHHIIIIIIICHDEEIGCEHIFHIGHIHGCHEHHHIIIIIHHHHHHCEHHHDEFHHEHFHHCHFEHIIIIIHIHIGHHIGECFGDHH=@.9BF.E.7
@SRR14933407.71/1
CGGCGATATTAATAATTATTATACCCTAACTTTCAATATATCAAACCATTTAACTTTAACATGCTTATACTCTAAATATAGCACTTAAGCATCATTTTTATAATGAAAATGAGTAAATTTTAATTCAATCCTGGAAAATCTTGTTGACGTAACGCTTCATAAATTAATAACGCAGCAGTATTTGATAAATTTAATGAACGAATATGTTCACTCATAGGAATAGATCGGAAGAGCACACGTCTGAACTCCAG
+
DDDDDIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIGIHIIIIIIIIIIGIIIIIIIIIIIIIIIIIIIGIIIIIIIIIIIHIIIIIIGIIIIIIIIIIIHHIIIIIIIIIIIIIIIIIIIIIIIIIIHHIIHIIIIIIIHIIIIHIIIGIIIIIHIIIIIIIHIHIIIIIIIIHIIIIIIIIIIIIIIIHIIIHHIGHIIIIIHHIIIHHIIIIIIIIIIIIIIHHIIHHIIIHIIIIIHIFHHIIIIG
@SRR14933407.72/1
CTCTGAAAAGAAAAAGTATAACAAAGATGTTGAAAAACGAGAGAAAGATTACAAAGCTTATTTGGATAATAAATCTAAAGAGATTAATAAAGCGATTAAAGCACAACGTTTTAGTTTGAATTATCATTATCCAACGGTTGCTGAAATTAAAGATATCGTTGAAACGAAAGCACCAAGAATATATGAAAAAACATCGCATCATCACGATTTCTTACATTATAAGTTAGGTATTGCGAATGTAGAAAAGTCAT
+
DDDDDIIHIIGHHIIICHHIHHIIGHHEHDGEHIIIIIIIIIIIIIHGHIHHIIIIGHHFGHIIHIIIIIIHIHIHIIHIIIIGHHHIIHIIIIGHHIIIGEHIIIIIEEHHHIFIIHIIIIIIIIHIIIIIIIIIIIHHHIIIIHIIHIIIIIGIIIIHIIIHIIHIHDHIIHIHIHHII?HHIHHIIHIIIHIIIHHHIIIIIHIGIHHE?BHGHHHFEHFCHHHEGHIGHF@D,?GHGHF.FAFGHHA
@SRR14933407.73/1
ACAATGATACCTTCTGACTAAAGCTGTAGTAACTATACTAGAACGTCTAGTTCATAAACACGTTTCACTCTATTCTTTATTTAAATAATATATAAGCAGCAAACGCCAAGGCTAATATACCAATAAAATAATTAATAAAGATTTTTATTTTAGTTTTTGTAATAGTTGACAAGTTTTATCACCTCTTAACATCGATAAATTAAACGCCGTTATTTTTCCTCTGCCTATCTTTTATCATTTCAGAAGGTACT
+
DDBDD?FGHIIHHIHHIHIIC1GE@?GHIHHHIFEHHHEH<<FEHFHHIHHIIHH@GHHIIG@FEHICHHIHHHHHGHHEGFHHHGECHHHIIHGHIIEHIFHHGCDH<G@HHHIHHHHG?<<<FGGEHHIEFCHGGHHHE?GH@GHIEFHIIHIIIIHHHGHHHHH1DHEEHHHGEHIICHGCCHHFGFEEE=GH@ECHEHGHHGIICDHHIIHHC@C@.DEEC?.FHHHIIIHIIIIFH..8G?GEHC.
@SRR14933407.74/1
GAAGTATTCACGCGCTCTTTGTGTCGCTTCTTTATCTTGTCCAGCAAATACTGTCACTAGACGATCAGGTAATACGTCATAATGTAAAGCATGTGATGCTGCTGGTCTTGCGATACCACCTGCACAACCACATACAGAATTGATCATAACTAGTGTTGTACCATCTTGTTTAAGAACTTTGTCAACATCTTCTGCAGTAGTTAATTGCTCATATCCCGCAGATTCAATTTCATTCCTTGCTTGTTCTACAA
+
A@BD@HI?@C<CFHCH=HHIH<1DCCFCEEHHEH@FHE<G1<F1@<<FE@EHEGHHHHCFHIIDH?H@1FFEH@CGF@0<G1CC<<CC1CCH1G11<1CG11<CFH@C@<1FCGHCCCGEH@EHHIHHECEGHC?<11<CC1C?CEEHEEGC?GECCCCG@G?F@C@0DHHG@GCFFH/<FHE/<ECH@@FFH?/<FEE?@FFHHIGEHEH.DFH?CDHGDHIHFH?GHHH?AG?FHHHHHIHHE.F?EB.
@SRR14933407.75/1
CCTATCGTTAATACACGTGTGTTAGCTAAAGATACATCATCATCTAAACGGAAGTACACACTTCCTGACAAACTATGTTGATGATCGATGACGATACCAGTTAAATTAGACATTAAGGCAAACAAACTGAAAATAATACATAATTGCCAACGTGCTTTCCATGTACGTCGACGATTCATTAAGTTTTTAAAATATGGAATATTCATCAACACATAGGCCAAAATAATAATTAAACCTACACGCTCAAGTAG
+
DDDDDIIHHIIIIIIIIIIIIIIIIIIIIGIIIIIIIHIIIIIIIIIIIIGHIIIHHIIIIIIIIIIIIIGIIIIIIIHIIIIIIIIFHFIIIIIIIIIIHFHHIIIIIIIIIHIIIIIIIIIIIHIHHIIIIIIIIIIIIIIIIIIHIIIIIIIIHIIIHIIIGHEHIIDHHHCHIIHHIIIHCHHHIGHIHIHIIHIIIIIHHIHH?HFHIIIIIGFHEHHHIGHIFHEHH@HHHI?HHGIHDHHCEH.
@SRR14933407.76/1
TGTTTGACTATCCAAGTCTTTACGGTCAACCAAAAAGATAACTTTCTTAATGTCATCTTGTTGTGATAAAATCTGACTCGCTTTAAAAGAAGTCAACGTCTTACCACTTCCCGTTGTATGCCACACATATCCATTATTCCCTGTCTCAGTCGCTTGTTGAATAAGTGCTTCTACCGCATACACTTGATACGGACGCATTGCCATCAGTATTCTATCTGTTTCATTAATAATCATATAGCGTGATATCATCT
+
DDDDDFEHIIIIH@HIHIIHIHHHHIIHIHIIIIIIHIIIIHHIIHIHIHIHIHHHHHIIHFHHHHIIIGHIIIIHIHIIGIHHHIIIIHIIIHHHIIHIH<FHEHHIIIIIIIHGHHIIIHIIIIIIIIIIIHHIIIIIHIGHHIIIIIIIIIHIHHIHEHHGHIIIHHHHIIHIIIH0CHHHIH<FEHHHGIGIHH=HHIHIIGHIIIIIH?HHHEHIIIIIIHEH?AFHIIIII?GCHEHFHHHEGHA
@SRR14933407.77/1
CCTACATCAACAACCAAACCACCTTTAACTACTTCTGTTACTTTCGCTTCGATGATTTCATTATTATCTAATTTTTCTTGTAAATAACTATAAGACTTCTCAGTTTCAAGTTGTCTTCTAGATAAGATGTAAGCTCCAGTTTCATTTTCTTCATCAAACTCAACTTTAGTGACATATGCTTCAACTTCGTCGCCCTCTTTTACAACTTCACTTGGGCTATCAATATGATGCGTAGATAGTTGACTAATAGG
+
B@DDAHHIHHHFHEEHHIHFIHFIIIIIIIEHHIIIIIIIHIIHEC<EEHIGE@1@CFHFH?CFEEHEHHIIIIIHIHC@GHFHEHIHECHHIH@GHHFCEDGGCGHIIIIIIIHHIHIGHEHH1<@FHHIHHIIHEHHEGHIIIHIIFHCEHHGIEHHCHHIHEHIHIECCGHICCGHEGGFHIIIIEGHEHII<HHHEH?HHHIIHHH?C?H/FHHIHHIGHIIHGIIHHIIHEEFFGHGHGHGCEC@G
@SRR14933407.78/1
TGTTAATAACGATGTAAAAGCTACATCGATGTCAATGTTATTTTCTCTTACTTTTTCACCTGCTCTAGTCGCTTCATTAATACCTTGTTCAGATAAATTAACATCTTCCCATCCAGTAAATAAGTTTTTAGCATTCCACTCGCTTTGTCCATGACGACATAAAATTAATTTTGGCATAACTTATAACCTCTTTCCTTTAATCTTTAGGACGTTATTTCAGGGATATTTTAAGATGATTTAATCTTAGCAAT
+
DD@DDIIEHHIIHIIHHIHIIIIIIHHHGHIIIHIIIHIIIIEHIHIIIIIIGHHGGHHHIIHHHHIIIIIHIGHHEHIIIIIIHIIHIIIIIHIHEHHIGGHHHIIHHIGIIIIIIIIIHHIGIGFHHIIIIIIIIIIIHGIGIIIIIHHHCHIGIIGHHHEHHHHGEHIIEHIHHHIGIIIIHIIGFHHHIIEHIIIEHICHIHEEFHHHIIIIEEHIFEGHIIIIIGEHFHHHHHHIEHHHIHHHHFH
@SRR14933407.79/1
AATTAAACCATGCTGACCATCGTCTCATTATTTATAAAGATATACATGAAAACATTGAAGATGGTATCACGTTGTTAATTGTTATGGCCGTAGTTCTTGTTTTACTAGTAATATTTGGTTTCATTAGTGCAGATAATATGGCTAAACGACAAACAAAAGATATCGAAACGATTATTCAAAAAATTTACTATGCCAAAAATCGTCATCTAGGTACATATACGCCTTTAAAAAACAACAGTGAACTAGAAGAA
+
DDDDDIIHHHIIIIIIIIIIHHHIIIIHHIIIIIHGH?GHHIIIHIIIIIIIIGHHHIGHIIIIIIEHIIIIIIIIIIIIIHHIIHIIHHHIIIHHIHIIEGHHHHIIEGFHGHHEHIHHIHIIIIIHGHIIIIIIIIIIIIIHIIIGIIIHIIIIIIIIHHIIE@HHHHIIHIIHGFHHIHIHGHIIIIIGIIIIHGHHEGGGGHHIIHHIHEHHHHHHHGCEGHHHHFF--BGHIE@.BFHIFHHEG@H
@SRR14933407.80/1
TATAAAAATCCAGATCCAGTGACAATATAATCAAAGCCATCATCTGTCTTTACAATTTCACTTTGGTATAATGTTCTAACCTTGCTTTCTACTTCAGTTTTTTGCGAACAAAAACCCGTAAAATCATGTGTACCTATGAATTGTTGTGCCGCTCTGTTCATTTTGTCTAAATCCAATGGTTCAGGAATAAATGTTTTCAAACCACTTTGAAATGGATCGCGTTGTTGTGCTTGATATACTTTATATAGATC
+
CDDDDIIIIIIIIEHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIGHIIIIIHIIIIIIIIIIIHIIHI<EEHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIHHIIIIHIIIIIIIIIIIIIIHHHIIIIHIIIHHIIIIGIHHIIIIIIIIIHIIIIIIIIHHHGHIIIIHHGCEHEHIIHHCHHIIIHHIIGHIIIHHIIHIHHHFHIH
@SRR14933407.81/1
ACAAACGAAATCTAAATTGGGTACTTCTGAAGCAAGAAGTGCTGTTGATTCAGTAGTTGCAGACAAATTACCATTCTATTTAGAAGAAAAAGGACAATTGTCTAAATCACTTGTAAAAAAAGCAATTAAAGCACAACAAGCAAGGGAAGCTGCACGTAAAGCTCGTGAAGATGCTCGTTCAGGTAAGAAAAACAAGCGTAAAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCT
+
DDDDDHIIIIIIIHIIHIIHHHIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIGHIIIIIIIHIIHIIIIIIIIIIIIIIIIIIIIIIIIIIEHIIIIIIIIIHIIIIIGIIIIIIIIIIIIHIIIIIIHIHIIIHIIHIHHHHHICECHHIIGGEEHIIHIIHHHHHHIIIIGHHIIIIIIGIEHHHIIIGHHHHDHH-.9.
@SRR14933407.82/1
GGGATATGACCCGTGTATTTTGAAAAGTGGTAAGCAATGTTTTTAGTTTATAGGGATTCATTAGTTGTATATAATAAGCATGAAAAGCATATAGTCGATCTTATACTTTTAATTGAGACTTATAGTGTATTTAAGTTATAGATAAATTCATTAAAATATTATGGAGGAATGAGTATGTCAAAGCGTCTATTATTGTTTGATTTTGATGAAACATATTTTAAACATAATACAAATGTAGAAGATTTAAGTCA
+
@@@@DFHGHHCH?DEC<C<<1FC<@@DFC1DGHH1FE@GEC?CE?111DGCEHHC11<C@HFG1<1<1D<DHHIEHH11FFC?HEH111GG@F1<D<1<<D<FHG11CG@@FHEG?G1<DFEHC@<<<<GGC1DCHHEHEFE1<FHFGHEGHGHFG@C<CEC?111C0D@G1<<FHHHH1FG?@CHH=EEHHHHH@FF?C@EH00GHHIIIF?/<<CGEG/<CG?CEHHIHH<FH/</C.?77.78GE@FC
@SRR14933407.83/1
TTACCACGTTATAATAAAGACGGTAATATGATTAAATGGTCACGACAAAAAGATTCTTTCTATTATTTCAATCCTAATTGGCATATTGTAGGTATTACATATGATAGTTTAGAGGAAATTAAGGAGAAATATTCAGAGCAGTTTTTAAAATACACACGTATAGTCAAAGTCAAACATGACTAATAATATATGGTAGTTTTTGAAAAGAAGTAAGGAAAGATTAAAAATTGCATATTTGAATAACCGTATAA
+
DDDCDIIIHHIIIIIIIIIHIIIHIHHIIHIIHHIIIIIIHIIIIIIIIIIIEEHIIIIIHHHIHIIIIIIIGHIIIIIIIGHIHHIHIHGHIIIGIIIHHDHIIIIIIIHIHHIHIIIHIHIHHHIIIIIIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIHHHIIHIIHHGIIIHIIIIIHIHEHIHIIHIEHHHG@GGHDGEHF?CHHHHHHHFCDCHIHHEHHHIIIIHIIHIHHHIIHIIEH-..;F
@SRR14933407.84/1
TTCGTCAATATACTTATTCGTTCTACTAATAAACTTCCATACCGTAGATAATGCCACAGAAAATTGCAAACTTTCCATGCTTTCAGTATAACTTTTCACTGTTTCTAAAGCCATAGCTTCCATTTCTTCATCTAATTCATGAAGTGGACCTTGATACGCTGGTAATTCGCCATCAAAGTACTTATTAATCATAGAAATCGTACGGTTTACTAAGTTTCCTAAGTCATTTGCTAGATCGAAATTTGTACGCT
+
DDDDDIIIIIIIIIIIIIIHHIIIIIGIIHIHHIIIIIIIIIIIIIGIHHIIHIGHIIIIIIIHHHIIIIIIGIIIHIIIIIIIIIGHIHIIIIIIIIIIIHIGHHHIIIIIIIIIIIIIIIIIIIIIIIHIIIHIIIIHHIIIIIHHHIIIIHIGHHIIICHHIIIIHHIIHIHIIIIHHIHHHIGHHHHHHHHHHIIIIHIDHECHIHIHHHHHHECGHHHHHIIIII?FHIIIHICHEHHEEHHIHC.
@SRR14933407.85/1
TGTGAGGCGTGAAAAGACGCCTCACATATTGTCTTGATTTATCTATCTTGGACATTTGAGGTTGTCGTTTGTTATGAATGCGTTTCTCAGCGTCAAATGTGAACTCTATCCATTCAATTTTACGCCCTTTTTTCGCTTTGATTTTATTGATAGTAAGATTATTAAATATAGAACCTAATTCAATAATAATAGGTTTAAAAACATTTTTGTTAATATCAGTCATACGATAAGATTTAGGTATATCTAAACGA
+
DDDDDIIIIIIIIIIIHIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIIIIIGHIIHIIHHIHIIHIHEHHIIIIIHIIIIIIHIIIIIIIIIIIIIIIIIIFHHIIIIGIIIIIIIIIIIIIIIIIIIIIHIHIGHHIIIFHHIIIIIIGIIIIIIIIHIIHIIHIIHHIHIHIEHHHHHGIIIIIIIIIIEHHIHIIIIEHHHIDGHHIIHHGFHHHHIGIIHIHIHGH@BH?EFHHIHIFHHH?EE.A9
@SRR14933407.86/1
AATTAGAAATGAAGTTATTCGTGCAACGTATGAATTTTTCAACAAAGATGGATTTACAAAGGTTGATCCACCAATTTTGACAGCAAGTGCACCAGAAGGTACAAGTGAATTATTCCATACTAAATACTTTGATCAAGATGCGTTTTTATCTCAAAGTGGTCAGTTATACTTAGAAGCTGCGGCAATGGCACACGGAAAAGTATTTTCATTTGGTCCAACTTTCAGAGCTGAAAAATCAAAAACACGTAGAC
+
DDDDDIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIHIHIHIIIIIIIIIIIIIIIIIIIFHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHIIIIIIIIIIIIHIIIIIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIHIIIIIIIGIIHHHIHIIIIIGIIIIIIIIGHIHEHIHIIIIHIIIH
@SRR14933407.87/1
GGACGTAAAGCACTTTTAATAGGGGTAACTATTGGACCTGGTAATAACAGACATCACTCAATTTATTCTATCGGCCAAAGAGGTGTTAACCAATTCTTAAAAAACATTGCACCTCAAGTATCGATGACTGATTCAGGTGGACGTGTTAAACCGTTACCAATACAGAACCCAGCATATCTAAGTGATATTACGGAAGTTGGTCATTACTATATCTATACGCAAGACACACAAAATGCATTAGATTTCCCGTT
+
B@@BDIIIIIIGIIIIHCHHFGHI<GHHHFHIIIGHHIIIIIIGHHIIGHH@FHIIHHHHHIIIIIHIIIIGIIIIIGIIIHGHHHHIIIIIIHHIIIHIHHHIHIIHIHIIHIIIIIIIHIIHHHIIGIHIIIIIIHHIIIIIIHFHIIIHIHIIHCEHIIIIIHHIIIIHIIHGHIHGCHIICGHIIEEFIIIIIGHGHIGHCHIHHCHHHHFAHIFHEHDHEHFHIEGHGHHIIEHIG..BBGH?@GG
@SRR14933407.88/1
GAAACAATTATTTGAACATCGAGGGTTACCACAGTTACCTTATATTAGTTTCTTACGTTCTGAATATGAAAAATATGAACATAACATTTTAAAATTAGTAAATGATAAATTAAATTACCCAGTCTTTGTTAAACCTGCTAACTTAGGGTCAAGTATAGGTATCAGTAAATGTAGTAATGAAGTGGAACTTAAAGAAGGTATTAAAGAAGCATTCCAATTTGACCGTAAGCTTGTTATTGAACAAGGCGTTA
+
DDDDDIIIIIIIIIIHHIIHIIIHIHHIIHIGIIIIHIGIIIIIHCHIIIIIIIIIIIIIIIIIIIIIGIIIIIIIGIIHIIHIHIIIIIHIIIIIIHIIICHHHHIIIIIIHHHHIHHHHIIHIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIHHIHIIIIHHHIIIIIIIHHHHHHHGIIIIIIIIIGHIHIIIIIHIIIIHIIIIIIHIGHHIIIIHIIIICHIIHHHHHHCGHHHIIHHIHHG
@SRR14933407.89/1
CTTAACACTTTTGAAAGGAAAAGCCTATTGTGATATCTATTGCAAACGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGCAATAATTAATTTAAACAATAAAAAATAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGCTCATTAGGCGACATGTTCCTGCATGTCGTCTTTTTACATTT
+
@DD?@11F<<<<F@CC@FHII1D?CG1<DHCHIHHEHFHIGC@@GEG=<CG@@<CGFEHI1FFF1<D<<1<<E0DG?1DCF@11<<CHHHHHHG11C1111<<<<1<0C/<<<C@111CC@@<1<FHEC@GFHDH@<<DHHHCC@H?EFHHHGHEI?C1G@?E0@<<<DGIE0<<FE<DFDFHHIE//DC@<GEHH/<DHE/GH/CE-.:87CHHGE@7-,5,.C...FEHHHH.B.FA8BEEHHCHH?HH
@SRR14933407.90/1
ATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTAAACCATCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCATTGATCCTTTATGACGTTCACGTTGTACAATGGCATTTCTTTCAGGCA
+
DDDDDEHIIIIHIHGHHHIGIIIIIGIEHIIIIIGIIIHHGFHIIHIHIIHHIIIHIGIIIIHIIIIIIIIIIIIIIIIIGIHHHEFDFHHFHHHHHHHHHHHHIIIIIIIIIGIIIIIIHIICGEHGHHHHHIEHHIIIIIIIIIIIIIHIHIIIIIIIHHHHIIIHIHHHHIHHHI?CHEHHHHIIIH?FHIII=@@D@EHCHEHGIHGHHHICHHDCCGHHIIIHHHFHIIIGHHFF?BAGG.@@BE.
@SRR14933407.91/1
ATTAGAATCTGGTGCAGAAGGTACGCGTGTAGAAGATACCATGACACGTATTGCAAAAAAACTTGGTTACAGTGAAAGTAACAGCTTTGTTACAAACACTGTCATCCAGTTTACGTTACATTCGGAATCGTTTCCTAGAATATTTAGAATTACCTCTCGAGATACAAACTTAATAAAAATTTCTCAAGCTAATAAAATTTCGCGTCAAATTACAAATAATGAAATTTCTTTAGCCGAGGCAAAAACACAAC
+
DDDDDIIIIIIIIHIIIIIIHIIIIIIHHIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIHIIIIIIIIIIIHIIIIIIIIIIIIIIIGIIIHIHIIIHHIIIHIIIIIIIIIIHHHIHIHIIIIGIIIIIIIIIIIIIIIIIIGIIHGHIIIIIIIIIIIIIIIIHIIIIIIHHHHIIIIIHHHFHH?HIIIHHHIIHIIIIIIIIIIIIIGHHIGIIHIIIHIHGH@EEHCD,<CHH.BGHIHHHH
@SRR14933407.92/1
GAAGAAGGAAAACTTGAAAAAGAAAAGGTAAGAATTAAAGTCGAACAAGATCCTGGTGCTTTAGGTATGCTAGGTACAAATCAAAATCAGCAAATGCAAGAGATGATGAATCAATTAATGCCTAAAAAGAAAGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACATGCCCTAATACAAGTGCATAACCACCACCATGC
+
DDDDDIIIIIIIIIIIIIIIIIHHIGHHHIICHIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIHHIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIFHIIIIIIIIIIIIIIHIIIIIIIIGIIIIIIIIIIIIIIIIIHIIIGIHIGIHIIIIIIIIIIIIIIIIIIIIIHIIFFHIIIIIIIIIIIIIIIIIHIIIFHHHHIHIH----....---.-7-..9-.---6--------;---.7.
@SRR14933407.93/1
ACCCCTCATTATAATCATCCTTATTTTCTATTTTTAAAAAGACAATTAGACCGCTCTTTAAACTATAGATTAATACTTAAGTTAAACTCATACATACTGATACCATACGTTAAATCTAACAATTTAAAATTCGTTATAACTATGGATTAAAGAGCTGCCCAACTCATTTAATCCTTAAAAACTTCACATGTGATTGTTTATTAATCCCTCCTTTATCTTATTAAATATCCTTATAACCCTTTTAAAATTAA
+
DDBDDIIIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIHIIIIIIIIIIHIIIIIIIIIHGEHHHHGHIIGIIHIIIIIIIFHIIIFHHHIIIIIIIIIIIDEHIIIIIIIIIIIIIIGIIIIIIIIICHHIHHHIIIHIIIIIIHHIIIIGIIIIHIIIIIIIIIIIIIIIIIIIIIDHHIIIIIIIIHIIHHIIIIHHEEHHIIHHHHIIIIEGHIHEIHIIGHHHIGHCFHIIIIHHEHHHICEHHHE
@SRR14933407.94/1
TAAACGTTTAGTTAAAGTGCTACTAACGATTTTAGTGATTGTGTTTTCAATTTTACTAGTAGGTGGTTGGTATATTTTTAAAAATGAAGCACCTCGTCCAACAAAGATAGTAGATCAACAAGGTCATACGCTTGTTACAAAAGGCGAGCTAATCAGTGGGCAAGCGATATATGAGAAATATGGGTTAACAGATTATGGCTCCTACCTGGGCAATGGTTCCTACTTAGGCCCAGATTATACAGCAGAGGCAT
+
CDDDDIHIEHHCGHIIIHIIIGIIHIIHHHIHHIIHGHHIIIF<FHHIIGIHIHIHIIIHIIHDGHEEHHIIIGHFHHHIIIIIIIIICHIIIGIIIIIGIGHHHIHHIIIHIHHIIHIHIIHHHHIIIGCEEHIIIIIEHHHHIIIIIDHIFIIIIIIGHIIGIGCHHHIIIIIIIIIIHHEGHIIIIIIIIHIFHHIIHIIIGGIHIIIGIHHHIIIIHIGIIHIIICHIIGHIHIIGCGCCHEFEGEB
@SRR14933407.95/1
CCAATCACAGAATGTAGTGGTAATGAAATATGTCAAGAATGGCTATATCACTTAGGTGTACCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACGATTCCTGTTTATATGCCATATATTACCTCTTATTTCATGACGCGTGCTATCGGCGACAGACCTTTAGTCGTCCCGCATCAATCACAAAATTTAGCATTTATTGGTAACTTTGCAGAAACAGAGCGAGATACTGTATTTACAACAGA
+
<0DD@EHHEF?1C<CHEF@?FHIIEHH@1<D<DCGHGEH1C@EFHEHHIGEEFHIGHH@E@CHHH1C<FH1CGHE1FH?H?HHIICHIHIHEHHC1@@GHIHHHGGH?GGHIIIIHHCHCHEEHEEFHFHEHEHHIHIIIHIIE?FGCDHHCE@EH=CCEDHC??<<C@0D<DC=HHH/<E-CEHFHIH?@HHIHCFE.@GHFHHHHIEBF.AB....BGHE.B@G.B?H?D78FHF@GFFCEHEHEC@HC
@SRR14933407.96/1
ATCGAGAATCTAGTCTTGATTTTTTAACCTCATTCTCTTCTATAGTATGCTTTAAAAAACGAATATCATTGTTAACATCTGATTGCTCTGACATTAATGTATAGTATTCGTTTTTAATTTCTTCCAATTTTTCATCGTGTGCTTCGTCTGAAACATATAGTTGTTCTTCAAGTTCACGAATGACAGCATTGAGTTCTTTTTGTTTACTTTTCAGAGACTTATAAGTATCTTGAGCTTCAGAAATCTCACTT
+
DDDDDHIIHIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIGHFHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGIIIIIIIIIIIIIIIIIHIHHHHIHIIIIIIIIIHIIIIIIIIHIIIIIIHIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGIGIGHHIIIIIIIIIGHIHIHIIIIIIIIIIHIIIIHIGHHHHHGHIHHIIHIIHGHGIIGHHFHHHHHIIHIE
@SRR14933407.97/1
GATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAAGAGATTAAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAACGTGTAGAATTCCACTTACATACTGCAATGAGTCAAATGGATGGTATACCAAATATTGGTGCGTATGTTAAACAGGCAGCAGACTGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCAGATGCTCACGCAGCAGCGGAAAAACA
+
DDCDDHHIHIIHHICHFEHH@HEEHIIHIIHIIIHIHHCHHHHHHHCGHGHHGGHIIIH/DCCDHHIIHHHHHCECGH1GHIIHHHIHIHEGHHIIFHIHH<FHHHHHHIIHIFIHHHIHHHIHIHHIHHHIIHIHHEHHHHHHIGHHHHEG@@DHGHF@GGEGHHHIIHICHHHHHIIIHCHHHHGIEHHFHHHHIHHH?@HHFGCF@HHICHIIIGCH?HFEGHHIFH?FHEHGHIIIFFDH?EHGH79
@SRR14933407.98/1
TATTTGAGTGCCATTTATGACTTAGGTGCTAAACGTATTGTTAGTTATGAGCTAGGCCCTTCTAACAATAATCAATTAGTATTTAAAACATTCAATCAAGCGATAGAAAAAGTGGAAAATACAAAAGGTATTTTATTTCATAGTTACCGAGGTTTTCAATATACATCGAAAACATTTAAACATATGTTGGATGAATGTGGCATGATTCAAAGTATGTCACGTGTTAGCAAATGTATCGATAACGGTCCAAT
+
DADCDGH1DHIIHIHIIIIIIIHIGHIHIIIIIIIIIIIIHIIIIIIIIIIIIIFEHHHIHIGIHHIIIHIIIIHFHHIH11<FFHIIIIIIHHIFEHFHEECEHHIIIIIIHEFHHHGHIIHIIIHHHIIIGIIIGHHHHHEHIIIICEEHE@HHGIIHHIHIIIHHHIIGHHEHHHDHHGCHE@HEC0F@0FHHHIHHF/FHC/<FHHHEHGHEHHHFGHHHGCHHIII..7.7C@F?G=.@CHG<FH.
@SRR14933407.99/1
AGAACACCTTCTAATGCAGCGCGAATCATATGTTCTTTTTTATGAGATAAAGTTAAACCGAAGAATGAACCTCTTGCATTTGCGTTCCAAAGCGGTGCACGTTCTCCAGCTAAATAGGGATGGAATATTAAACCATCTGCACCTGGTTTAACACGCTTTGCAATTTGAGTTAAAACATCATAAGGATCAACACCGAGACGTTTCGCAGTTTCGACTTCGCTTGCTAACAACTCATCGCGCAACCATCTCAA
+
DDDDDIIIHHHGDHHEHHI@FHIDCHIEEGHHE@HIHIIHIFHHHIHIIIIIHIHHIIII<EHHIIIHFHEHHFHHFHIFH11FFHE@FHEE?EHGHGHHIGHGH@HIIIICHHHHHHHH?CEHHIIIIIGEECHHHEHHIHIHGGGFEFHFHHCHHGHHEHHIIHHHIH?C@FHFHF?CHHHHFFE:GFHCG@?HHGIHHHEF@HHH@HIHHF=G=EHCHHHCCCB..;BEGHGC-5><HC,@ACB-9BB
@SRR14933407.100/1
CTGCTCTAAAATTTCTGTTTGTTGTCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGCCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAGCTGTTTTACTTACTTCTTGAACACGATTAACATCATCTA
+
DDDDDIIIIIHHIIIGHHCHIHIIIIIHIGIHIIGIGHIIHIIIIHIIIEHIIFHIFHIIEHHIHIIIFHHIIIIIIIIHGHIIIHIHIIGIIIIIHHIIIDGIIIIIIIIIIIIIIIIIIHIGHHIIIIIIIIIIHIIIIIIIIGIIIIIIIIIIIIIIIHFHIIIIIHHIIHIGHICHHEHHIHFEHIIC?GHHIHIIHHIIG.FHHEF@AHHH?GHFHHHFEHHCEHHEHHHHGHHHHIIGBGHHHGC
@SRR14933407.101/1
GGTAATCCCATAACTTCAGTTAATTCATAATCAACGCCACTCTCTTCAAGAGTCTTTTTAATAGTATTTTTAAATTGCTTTAATGGATATACACTTGCGTTTGTACATAATAATAATGTTCCTTCAGATGATAAGATATTTAAGGCGCCAGTAATTAATTTGTCATAATCTTTTTGCACTGAAAATATACGTTTTTTATTGCGTGCAAAGCTAGGTGGATCAATCACGATCGTGTCATAACTATGTCCATG
+
DDDDDIIIIIIIIHIIIIIIIIHIIIIIIIIIIIHIIIIHIIIIIIIIGHHIHIIIHHIIIFHHHII1DFHIHCFHIIHG@GHIIIGEHHHIIHHIHHIHHDFDHHHIIIHHHIIIIIIIIIIIIIIIIIHIIIIIIIHIHIIIHHIIIHIIGIIIHIGHHIIIIHICHIIIIIIHHHIIIHHIHHIHHFGDHHHIIIIICHHHIHHGFHHIGFFHHHHIIHHIIIHIBH?C@EHHIIIIIGIIFH?FH?.
@SRR14933407.102/1
CCGGAAAAATATTTAAATGTTGGTATTCAGGTATTAAATCAATAACGGTTTTTGAATGATGATCTTCAGTTGCCGATATATAACCTTTTGGCTTATTTAACATAATATAGACATTTTCAACGTATTCTATTAATTCTCCACGAACTGATATCTTATCGTTTTCTGGTTCTATATGTGTTTTTGGTGATTTAATTACTTGTTCGTTGACATTTACAAGGCCTTTTTTAAGTAACTGTTTGACCTCATTACGT
+
@D@BDIHHHIEEECG?F<C<<FF0DG<EEHEDH1FH?@H1<@GHHHHIDCHHFIHHHHCCGHEHIIICGEHIEGH/</CEC1CF1DDEFHDH111DDGFGH1CFCCDHFEHHHHHIHH?<CCHCGE@1<<C<CC1FHEHCC@EC<@CCDEEGCEF@EEFF11<CFC<1DFH@0D<CG<D<D<EE@GFF/</<DFEHII/F@GHIIGDHC?GFC@@HHCH?@DGHED?H?G?GHHHBG@FFHEEHHIHGC.7
@SRR14933407.103/1
TAACGGTCCAAATTCGGAAACATGTAACTGATGAACTTTTGGTAGTCCTATACGAATATAACCGTATCCGATGATTACCATAAATATGCCCAGTGTCATAATAATGTATTGATTTAAACGATCTTGCATAACACGTTTAAATCGCTTCGTAGTAAACTTTTCAAATTGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA
+
DDDDDIIIIHIIIIIIIHIHHIIIIIHIHIIIIIIIIIIIHHHIIIIIHHIIIIIHHIIIIIIIIIIIIIGHIICGHIIIIGHHIIIIIIIIHEHFHHIHHEHHHFHIIIIIIIIGIIHIIIIHIIIIIIIIIIHFHHIIIIIHIIHHHHIIIIIHIHHHIIHHHIIIIIHHI0EHGGDHHGH/GHIIGIIHIHIHIHHE@FHHIHHHHIHIFH7..BGHIHHIIHHGFH@.8@.6BE@EAHDHH?D,--7
@SRR14933407.104/1
GCAGCGATTTTACAAGTAGAATCAATCGTTAAAAAGCCAGTAGTAATTAATGATATGATTGCAATTCGAAGTATGGTTAATTTATGTATTTCAATTGATCATCGTATTTTAGATGGTTTACAAACAGGTAAATTTATGAATCATATTAAACAGCGTATCGAACAGTATACTTTAGAAAATACAAATATATATTAGTGATAACATAGATGCATCTATCGACAACTTGTTTTATCTTGTTCTTGTCGATGGAT
+
DDDDDHDHIIIIIIIHIIIIIIIIIIIIHHHIHIIHIIHGIIIHHIIIIHIIIIIIIIGIIIIIHIIIIHHIHHHIIIIIIGHIHHIIIIIIIIIIIIIIIGHIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIGIIIIIIIIIIIHIHIHIIIIIICHIIIHCHIIHHHIIIIHIIIHIIHHEHIIIIIHHIIIIIHIIIIIIIIEHIHEHIGHHHHHIHHHGHEHHHHHGIGHIIIIHIIIICGHH?
@SRR14933407.105/1
CCCGTTTTATAATTACTATTGTTGTAAAAAAGGTTAGCTAAGCTAACTATTTTGTCTTAGGAGATGTCGCTATGCTATCACAAGAATTTTTCAATAGTTTTATAACAATATATCGCCCGTATTTAAAATTAACAGAGCCTATTTTGGAAAAACACAATATATATTATGGTCAATGGTTAATCTTACGCGATATCGCTAAACATCAACCCACTACTCTCATTGAAATTTCACATAGACGTGCAATTGAAAAG
+
DDDDDHIIIIIIHHIIIIIIIIIIIIIIHIIHIHIIIIIIIFHHIIIGIIIHHEHHIHIIIIIIIIIIIIIIIIIIIIIIGIHFHIIIIIIIIIIGI@?FHDGHIIIIIIIIIIHIIIIIIHHIIIHHHIIIIIIIIIIGIIIHIIIIIIIIIIIICCFHIIIIIIIIHIIIIIIGGHIICHIIIIIIHIDHIIGIIHIHHHHHIIIIHIIHIHIIIHIFHEGHGIIIGIIEHIIHIGHHHIHII?EHIIA
@SRR14933407.106/1
GAAAGCTTCTGAAATAAATTCAGCTTTTGAAAAGATGAATAATGGCAATGTCTTTGTTGGTATTGTAATAGGTTTAATAGCAGCTTATGCATACAATAAGTTTAGTGAAACAGAATTACCATTAGCATTATCATTTTTTAGTGGTAAACGTTTAGTTCCAATTATGACTGCATTTTACTGTACATTTTTAGTTGTCATATTGTTATTCTTATGGCCACTACTTTATTCATGGATTGTAAAATTTGGTGAAT
+
DDDDDIFHIIIIIIIIIIIIIIIIIIHIIHIIIIIIHIIHIIIIIIHHIHEHIHHIIIHIIIEHIIIIIIIIGHIIIIIHHIIIIIIIIIIHIIIGGHHIEFHHHIIIIIIIIIIIIHIIIIIHHIIIIHIIIIIIIIIIIIGIHIEHHIHIIIIIIIIIHIGIHEHHHIIIIHHHIIIIIIIIIIIHIIIIHHIIHIHHHIIII0CHHIHI?HHCHIIIIIIIHHGF?GFFHHIEHIGHIHIIIEH@HGE
@SRR14933407.107/1
CATATGTCTTCGCAATAATATTCCCCATCGTTTCTCCGAGGTTACTTCCAACTGCCTCTAAGTAGTGGTCCGGTTGTCTTAATTCCAAATCATCAAAGAAAATTTGATTCAATGTATAATCATAATTTTGACCAGTGTGTACTACTATCTGATTAAAATATTGATCACATGCTTTAATCGTTGATGATAAACGAATGATTTCAGGCCTTGTACCAACTATTGTAATTAATTTTAGTTTTTCCTTGCGCTAT
+
<<0<D1DDCHCCGHCHIE?GH@HE?FHHHE<C<CCCFHIIE0@CEEHIHEHHII@@11CE<FHH?H11DHEHIHGHHEE?HHIIG1DGHH1CEEHCEHHHHHHEE1F@<CGHFFHFFD?FCHEE@EFG@EHIH@GCEE1<<DGH1<CG?1@HH?HFFCGCFE1CH@GHHHCHHHGH<DD0<0<C?0CCGCH?/@@C@/<//DG/?/<CDG@GEHHICEHH.7:..;DF?G7.CGCG8.BEG..9..,5?-9

Scientists will deposit FASTQ files into NIH databases

GeneBank for genomic sequences
Sequence read archive (SRA) for sequencing data
RefSeq for reference genomes
BioProject for curated resources for a specific project
Many more

After today, you should be able to

1. Explain the basic concepts and importance of genome assembly.
2. Interpret FASTA and FASTQ file formats and their role in storing sequences.
3. Perform and interpret quality control on reads using FastQC.
4. Identify common quality issues in sequencing data and explain their impacts.
5. Describe the process and importance of sequence trimming and filtering.

Per base sequence quality

Poor

Good

Excellent

Box and whisker plot of base-call accuracy

Per sequence GC content

Strong deviations from normal distribution could indicate contamination

Activity: Quality control

TopHat Questions

After today, you should be able to

1. Explain the basic concepts and importance of genome assembly.
2. Interpret FASTA and FASTQ file formats and their role in storing sequences.
3. Perform and interpret quality control on reads using FastQC.
4. Identify common quality issues in sequencing data and explain their impacts.
5. Describe the process and importance of sequence trimming and filtering.

Activity: Read trimming

With cleaned data, we can now assemble our reads into contigs/scaffolds

Before the next class, you should

Finish Assignment 01, which is due Thursday at 11:59 pm.

Lecture 04:
De novo assembly

Lecture 03:
Quality control

Today

Thursday