Goals:

• Capture meta-data
• Compare lots of results
• Improve library prep

Metadata

Lots of Libraries

Improving RNA-seq

• Senstivity, simple lab workflow, reproducible, reflect reality
• What is reality??
• ERCC (limited)
• Maintain distribution across input RNA amounts

Truth?

Transcript Abundance

Details matter

5'-3' Coverage

Aggregation Hides

What's this?