Basin-scale biogeography with  3-domain ASVs

What is the resolution of ASVs?

  • Are Pro and Syn indistinguishable?
  • Prochlorococcus ecotypes distinguishable?

What is the resolution of ASVs?

  • Are Pro and Syn indistinguishable?
    • Yes, sometimes, but rarely
  • Prochlorococcus ecotypes distinguishable?
    • Yes, for most ASVs

What is the resolution of ASVs?

  • New databases:
    • SAR11 ecotypes
    • Phytoplankton 16S / 18S
      • Link traits such as size to ASVs?

Pančić and Kiørboe, 2018

ASV phylogeny

P16N/S

16S phytoplankton (EUK vs PROK)

Samples courtesy of Craig Carlson

P16N/S

16S phytoplankton

Samples courtesy of Craig Carlson

P16N/S

16S minus cyanobacteria + mitochndria

Samples courtesy of Craig Carlson

P16N/S bacterioplankton

P16N/S bacterioplankton

P16N/S bacterioplankton

P16N/S bacterioplankton

P16N/S bacterioplankton

P16N/S bacterioplankton

Next steps: Network analysis

plastid

16S

mito 16S

nuclear 18S

Space

Abundance

A eukaryotic phytoplankter

(Artist's impression, not real data)

Next steps: CMAP

  • Assist the Armbrust group ingesting GEOTRACES chemical data into CMAP
    • Manual curation required - Yubin and I are working with Tansy, Diana and Rhonda
  • ASV data to CMAP
    • GA03
    • GP13
    • GA02
    • GA10
    • P16N-S
    • I8/I9
    • AMT
  • nifH qPCR vs amplicons