Norm to [DNA] + amplicon abundance = semi-absolute abund per taxon
Section across poles showing community changes?? 3-basin perspective
Craig Carlson, Elisa Halewood, UCSB
P16S/N
Fraction of 18S amplicon sequences
16S
plastid 16S
18S
Jan-Feb 2005
Feb-Mar 2006
With deep sequencing, good coverage for all 3 domains
With 200k sequences per sample, practical detection limit = 1 copy nifH / mL
Kendra Turk-Kubo, Rosie Gradoville, Jon Zehr, UCSC
AMT
Glen Tarran, Andy Rees, PML
nifH gene copies / L (x 106)
16S ASV relative abundance
Sensitivity & accuracy vs qPCR?
UCYN-A (AMT 20)