Figure 1: Overview of sampling and data processing workflow. Oxygen and sulfide depth profiles shown in blue and orange, respectively. SRF=surface, RXL=redoxcline, EUX=euxinic. 3DMB=3-domain metabarcoding, MAGs = metagenome assembled genomes, FL-16S = full-length 16S. OOI = organism of interest. FISH = fluorescence in-situ hybridzation.
[H2S]
[O2]
depth
EUX ~3°C
RXL ~10°C
SRF ~22°C
DNA
-3DMB
-MAGs
-FL-16S
OOI
(function,
microdiversity)
(broad group level,
microdiverse subcluster level)
FISH probes
OOI-specific measurements:
-Growth rate
-Respiration
-Resilience
Field sampling
Lab processing
Field incubations