Lab 9

Lab 9

 

  1. Turn in Lab 8 by the staplers on the North bench.
    1. Needs a name and group number
  2. ~10 min.: Q&A on Lab 8 material
  3. 20 min.: Quiz 8
  4. ~2 hrs.: Lab 9: Hematology
  5. ~30 min.: Next lab reminders/questions/review

Lab 8 Q&A

Langendorff Experiment

Neurotransmitter/Drug effects

  • Adrenaline (epinephrine)
    • Sympathetic
    • β1 receptor activation
    • Propanolol - β-blocker
    • Phentolamine - α-adrenergic antagonist
  • Acetylcholine
    • Parasympathetic
    • M2 receptor
  • Verapamil
    • Voltage-gated Ca2+ inhibitors.
    • Reduces strength of contraction.
    • These channels are also found in SA and AV nodes.
  • Atropine
    • Antimuscarinic (AKA anticholinergic)
  • Strophanthin
    • Inhibits Na +/K + ATPase, inhibiting Na +/Ca 2+ exchange, increasing intracellular Ca 2+.
    • Increases contractility and vagal response

Quiz 8

Lab 9: Hematology

Instructions

Lab Purpose

To allow students to experiment with principles of hematology, blood typing, genetics and immunology.

Lab objectives

  • Give normal blood values for hematocrit, erythrocyte count, hemoglobin content and leukocyte content.
  • Be able to suggest causes of abnormal values.
  • Determine hematocrit values in whole blood & suggest causes for high and low values.
  • Determine blood typing for the ABO and Rh protein factor systems.
  • Explain heredity patterns for ABO blood types.
  • Determine appropriate blood transfusion matches given any blood type for both the recipient and donor.
  • In the event of insufficient matched blood types for transfusion, name cross-matched transfusions that could be done without significant antigen-antibody reactions.

Lab Safety

The capillary tube centrifuge

I will be dispensing the antibodies for blood typing.

Core Principles

Blood Components

Normal Blood Values

  • Hematocrit
    • ♂ 45%-52%
    • ♀ 37%-48%
  • Erythrocyte count: 4.5-5.5 million/microliter
  • Hgb: 14-16 grams/100 mLs
  • Leukocyte: 5k-9k/microliter
  • Platelets: 200k-500k/microliter

Blood Types

Erythroblastosis fetalis

Lab 10: ECG and Blood Pressure

  • Read:
    • Silverthorn pages 443-472, 482-496
    • Stanfield pgs 368-385, 419-426
  • Bring:
    • Wear exercise clothes
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