Mark Robinson
Supervisor: Carole Goble
Expresses Workflows
Container format
with metadata
Consistent feedback from CWL community on features and bugs found
Has been used to visualise workflows from:
and more!
In the form of direct chat and Github issues
Easy to run and make changes - MVC web application and configuration documented on Github
The spirit of reproducible research is sharing!
Allow CWL workflows to be visualised to aid understanding as to what their purpose is quickly
#!/usr/bin/env cwl-runner
cwlVersion: "cwl:draft-3"
class: Workflow
inputs:
- id: p1
type:
- "null"
- type: array
items: File
description: list of files containing the first end of paired end reads in fasta or fastq format
- id: p2
type:
- "null"
- type: array
items: File
description: list of files containing the second end of paired end reads in fasta or fastq format
- id: output_prefix
type: string
description: prefix for output files. will output prefix.aligned.bam and prefix.aligned.stats
- id: reference
type: File
description: "lobSTR's bwa reference files"
- id: rg-sample
type: string
description: Use this in the read group SM tag
- id: rg-lib
type: string
description: Use this in the read group LB tag
- id: strinfo
type: File
description: File containing statistics for each STR.
- id: noise_model
type: File
description: File to read noise model parameters from (.stepmodel)
secondaryFiles:
- "^.stuttermodel"
outputs:
- id: bam
type: File
source: "#samindex/bam_with_bai"
- id: bam_stats
type: File
source: "#lobSTR/bam_stats"
- id: vcf
type: File
source: "#allelotype/vcf"
- id: vcf_stats
type: File
source: "#allelotype/vcf_stats"
hints:
- class: DockerRequirement
dockerLoad: https://workbench.qr1hi.arvadosapi.com/collections/download/qr1hi-4zz18-x2ae13tsx5jqg8d/1nduktd8dpvhdpgsva82lje0i710kgzb6rttks5jldx7s2y7k9/7e0c0ae3bf4e70442f9b8eee816ec23426d9e1169a2925316e5c932745e21613.tar
dockerImageId: 7e0c0ae3bf4e70442f9b8eee816ec23426d9e1169a2925316e5c932745e21613
steps:
- id: lobSTR
run: lobSTR-tool.cwl
inputs:
- { id: p1, source: "#p1" }
- { id: p2, source: "#p2" }
- { id: output_prefix, source: "#output_prefix" }
- { id: reference, source: "#reference" }
- { id: rg-sample, source: "#rg-sample" }
- { id: rg-lib, source: "#rg-lib" }
outputs:
- { id: bam }
- { id: bam_stats }
- id: samsort
run: samtools-sort.cwl
inputs:
- { id: input, source: "#lobSTR/bam" }
- { id: output_name, default: "aligned.sorted.bam" }
outputs:
- { id: output_file }
- id: samindex
run: samtools-index.cwl
inputs:
- { id: input, source: "#samsort/output_file" }
outputs:
- { id: bam_with_bai }
- id: allelotype
run: allelotype.cwl
inputs:
- { id: bam, source: "#samindex/bam_with_bai" }
- { id: reference, source: "#reference" }
- { id: output_prefix, source: "#output_prefix" }
- { id: noise_model, source: "#noise_model" }
- { id: strinfo, source: "#strinfo" }
outputs:
- { id: vcf }
- { id: vcf_stats }
Enable easier sharing of workflows to aid reproducible research
compile.svg
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},
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"name" : "Gerrit Botha"
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"name" : "Michael R. Crusoe"
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"name" : "Milt Epstein"
}, {
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"name" : "ShakunBaichoo"
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"uri" : "https://github.com/grbot",
"name" : "Gerrit Botha"
}, {
"uri" : "https://github.com/ShakunBaichoo",
"name" : "ShakunBaichoo"
} ],
"retrievedFrom" : "https://github.com/h3abionet/h3abionet16S/blob/70498847fd6bfc63f44c59c748233cac7f8dd85d/workflows/uparseRenameOTUs.cwl",
"conformsTo" : "https://w3id.org/cwl/v1.0",
"bundledAs" : {
"uri" : "urn:uuid:8f1ef90e-5d98-4cea-9a14-cae28ccc8489",
"folder" : "/workflow/"
}
}, {
"uri" : "/workflow/uparseRenameWithMetadata.cwl",
"mediatype" : "text/x-yaml",
"createdOn" : "2017-02-27T11:48:59.456Z",
"authoredBy" : [ {
"uri" : "https://github.com/grbot",
"name" : "Gerrit Botha"
}, {
"uri" : "https://github.com/mr-c",
"name" : "Michael R. Crusoe"
}, {
"uri" : "https://github.com/mepstein-gh",
"name" : "Milt Epstein"
}, {
"uri" : "https://github.com/ShakunBaichoo",
"name" : "ShakunBaichoo"
} ],
"retrievedFrom" : "https://github.com/h3abionet/h3abionet16S/blob/70498847fd6bfc63f44c59c748233cac7f8dd85d/workflows/uparseRenameWithMetadata.cwl",
"conformsTo" : "https://w3id.org/cwl/v1.0",
"bundledAs" : {
"uri" : "urn:uuid:9658c5e4-6ec5-48bf-b3d2-c9fb0dcef87c",
"folder" : "/workflow/"
}
}, {
"uri" : "/workflow/uparseSort.cwl",
"mediatype" : "text/x-yaml",
"createdOn" : "2017-02-27T11:48:59.598Z",
"authoredBy" : [ {
"uri" : "https://github.com/grbot",
"name" : "Gerrit Botha"
}, {
"uri" : "https://github.com/mepstein-gh",
"name" : "Milt Epstein"
}, {
"uri" : "https://github.com/ShakunBaichoo",
"name" : "ShakunBaichoo"
} ],
"retrievedFrom" : "https://github.com/h3abionet/h3abionet16S/blob/70498847fd6bfc63f44c59c748233cac7f8dd85d/workflows/uparseSort.cwl",
"conformsTo" : "https://w3id.org/cwl/v1.0",
"bundledAs" : {
"uri" : "urn:uuid:8be968a6-a678-43d2-8a39-bfbf29a19451",
"folder" : "/workflow/"
}
}, {
"uri" : "/workflow/uparseTruncateLength.cwl",
"mediatype" : "text/x-yaml",
"createdOn" : "2017-02-27T11:48:59.814Z",
"authoredBy" : [ {
"uri" : "https://github.com/grbot",
"name" : "Gerrit Botha"
}, {
"name" : "Ubuntu"
} ],
"retrievedFrom" : "https://github.com/h3abionet/h3abionet16S/blob/70498847fd6bfc63f44c59c748233cac7f8dd85d/workflows/uparseTruncateLength.cwl",
"conformsTo" : "https://w3id.org/cwl/v1.0",
"bundledAs" : {
"uri" : "urn:uuid:4ab00aca-93dd-4006-adc4-7565ac4e3f20",
"folder" : "/workflow/"
}
} ]
}
bundle.zip
├── mimetype
├── .ro
│ └── manifest.json
└── workflow
├── completeWorkflow.cwl
├── concatFasta.cwl
├── fastqc.cwl
├── generateRReports.cwl
├── qiimeAddMetadata.cwl
├── readPair.yml
├── uparseChimeraCheck.cwl
├── uparseDerepWorkAround.cwl
├── uparseFastqMerge.cwl
├── uparseFilter.cwl
└── uparseTruncateLength.cwl
Allow the discovery of CWL workflows from different authors
(Almost done, should be in production before code freeze)
(Similar to workflows but have additional considerations eg InputBinding/Commands)
(Existing application is designed with this in mind)
(Proprietary organisations don't want to share! Though this is not a priority as we want to encourage it especially in research)
(Partially done yesterday, added support for private repos when running their own instance)