Theodore B. Davis, Richard D. Morgan, Bradley W. Langhorst
New England Biolabs
Is it possible for a small group of scientists to sequence and assemble a large eukaryotic genome as an evening and weekend project?
Goals:
Odocoileus virginianus borealis
[1] Martinsen, E. S., Mcinerney, N., Brightman, H., Ferebee, K., Walsh, T., Mcshea, W. J., … Fleischer, R. C. (2016). Hidden in plain sight : Cryptic and endemic malaria parasites in North American white-tailed deer ( Odocoileus virginianus ). Science Advances, (February), 1–8.
20X
28X
35X
N50 | 6Mb |
# of Contigs | 3449 |
Longest Contig | 33Mb |
Genome size (in contigs) | 2.5Gb |
Ted Davis - Initiative, Tissue, DNA Isolation, Illumina libraries
Rick Morgan - DNA Isolation, PacBio libraries
Brad Langhorst - Assembly, Annotation, RNA isolation, RNA-seq library
Images: https://www.flickr.com/photos/bullfrogphoto/2374256270
https://www.flickr.com/photos/lsmith2010/5950507945
https://www.flickr.com/photos/avexbhphotos/12332751825
Josh McCoy - DNA/RNA Source tissue