Segmentation problems

Molecules

DAPI

*Moffitt, J. R. et al. Molecular, spatial and functional single-cell profiling of the hypothalamic preoptic region. Science 362 (2018)

Segmentation problems

MERFISH

[1]

osm-FISH

[2]

1. Moffitt, J. R. et al. Molecular, spatial and functional single-cell profiling of the hypothalamic preoptic region. Science 362 (2018)

2. Simone Codeluppi, Lars E. Borm, Amit Zeisel, Gioele La Manno, Josina A. van Lunteren, Camilla I. Svensson & Sten Linnarsson. Nature Methods 15, 932–935 (2018)

Neighborhood composition

Segmentation across protocols

Segmentation across protocols

MERFISH gut data

Segment nuclei against cytoplasm

NCV

Compartments

Segment nuclei against cytoplasm

Baysor package