ethics in
digital image processing
what (not) to do!
Dan White, GE Healthcare / Fiji
NEUBIAS COST Action. NeuBIAS 2017 TS2: BioImage Analysis for Early Career Investigators. Oerias, Portugal. Tuesday 14th Feb, 17:00-18:30
What is scientific ethics?
Understand
Image Formation Process
systematic errors
are fixable
"raw, noisy"
contrast restored (deconv.)
image restoration
fix systematic errors
"raw, noisy
contrast restored (deconv.)
"raw, noisy"
Images: Alex Bird / Dan White MPI-CBG
measure and correct
systematic errors
colour shift
corrected
Bias
no cherry picking:
"the one that worked"
double blind assays
With power comes responsibility
Open
Reproducible
Available
Openness:
Share Tools
Open Access
Who Paid?
Reproducible
and Available:
Version Control
Share Scripts/Code
Share Image Data
Cheating is easy...
... to detect!
(and punish?)
Cheat detection
Cheat detection
copy
paste
Cheat detection
NYT Health (@NYTHealth) tweeted at 9:22 pm on Wed, Mar 08, 2017:
Forensic analysts say Ohio State's star scientist, who received millions in grants, manipulated images of his data
Guidelines:
Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114110/
http://swehsc.pharmacy.arizona.edu/micro/digital-image-ethics
Digital Images Are Data:
And Should Be Treated as Such
Douglas W. Cromey https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210356/
Guidelines 2:
Seeing is believing? A beginners' guide to practical pitfalls in image acquisition
Alison J. North http://jcb.rupress.org/content/172/1/9
The 39 steps: a cautionary tale of quantitative 3-D fluorescence microscopy.
James Pawley
Guidelines 3:
ImageJ Wiki:
Give yourselves
a chance!
a testable hypothesis?
The scientific method... dont spend too much time on
fishing trips and stamp collecting
an assay: measure something
Are your eyes reliable?
Give readers a chance!
No Red/Green = Yellow merge images:
colour blindness is common
use green/magenta or red/cyan
Ethics in digital image processing copy
By Daniel White
Ethics in digital image processing copy
Ethics in digital image processing
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