Jesse Bloom PRO
Scientist studying evolution of proteins and viruses.
Fred Hutch Cancer Research Center / HHMI
Slides at https://slides.com/jbloom/gs-retreat-2021
We can address this question by studying historical evolution of other human coronaviruses.
We chose CoV-229E, which causes common colds and has circulated in humans since at least 1960s.
old virus (e.g., 1984)
old virus (e.g., 1984)
extent of virus neutralization
Measure how old serum neutralizes old virus
old virus (e.g., 1984)
extent of virus neutralization
Measure how old serum neutralizes newer virus
newer viruses
measles-like
influenza-like
We experimentally generated CoV-229E spikes at ~8 year intervals so we could study them in the lab:
- 1984
- 1992
- 2001
- 2008
- 2016
We are studying basis of these differences, as ideally vaccines would elicit more evolution-resistant sera as on the right.
Plot of sequence variability across CoV-229E spike taken from Eguia, ..., Bloom, PLoS Pathogens (2021) . See also Wong, ..., Rini, Nature Communications (2017) and Li, ..., Rini, eLife (2019) for detailed structural studies of evolution in receptor-binding loops.
RBD
fluorescently labeled antibody
yeast
fluorescent tag on RBD
Click here for details on how library is made.
In maps, tall letters indicate strong escape mutations
Crowe lab (Vanderbilt): James Crowe, Seth Zost, Pavlo Gilchuk
Chu lab (Univ Wash): Helen Chu, Caitlin Wolf
Veesler lab (Univ Wash): David Veesler, Alexandra Walls, Ale Tortorici
King lab (Univ Wash): Neil King, Dan Ellis
Li lab (Brigham & Women's): Jonathan Li, Manish Choudhary
Whelan lab (Wash U)
Boeckh lab (Fred Hutch): Terry Stevens-Ayers
Alex Greninger (Univ Wash)
Janet Englund (Seattle Children's)
These slides: https://slides.com/jbloom/gs-retreat-2021
Adam Dingens
Will Hannon
Amin Addetia
Keara Malone
Tyler Starr
Allie Greaney
Rachel Eguia
Bloom lab (Fred Hutch)
Sarah Hilton
Kate Crawford
Andrea Loes
By Jesse Bloom
Genome Sciences retreat 2021
Scientist studying evolution of proteins and viruses.