detection of densely packed nuclei, together with signal correlation analysis from other channels
Data is 3D, but seems like there's no overlaping of nuclei in the axial direction
XY
YZ
XZ
This allows us to work in a Max projection
DAPI nuclei at 10x
DAPI intensities
Target intensities
for a threshold of 500, 28.74% of nuclei are active
By Felipe Delestro