Imaging Mass Cytometry

10/12/2019

lab meeting

Fluorescence imaging

• sample genetically modified to express fluorescent markers (allows imaging of live cells)
• use of antibodies with fluorophores

we get a good spacial resolution, but we're restricted on the number of markers

mass spectrometry ≠ mass cytometry

NASA's Phoenix Mars Lander analyzing a soil sample from the "Rosy Red" trench with the TEGA mass spectrometer

Single Cell mass cytomety

cross talk is practically innexistent

we get a lot of features, but we completely lose the spatial information!

Hyperion imaging system for

Imaging Mass Cytometry
(IMC)

Nature methods, 2014

Up to 37 markers int the same sample

https://www.fluidigm.com/hyperion/interactive-demo

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