Investigatory Project
Why this topic?
3,000,000,000+
People at risk of malaria and dengue
640,000,000
People infected with Malaria, Dengue, and Yellow Fever
2,700,000
Deaths every year due to Malaria
Philippines
is at
risk
Larviciding
=
mosquito population control
=
reduces number of disease carriers
=
less risk, less infections, less deaths
Larviciding
=
expensive
=
not easily accessible
=
damaging to the environment
what if
there's a larvicide that is
cost-effective
easily accessible
eco-friendly
?
Problem
Citrus aurantium juice+peel
Cymbopgon+H2O+Alcohol
Palm-based cooking oil
=
Death of larvae
?
Hypothesis
If
Citrus aurantium juice+peel
Cymbopgon+H2O+Alcohol
Palm-based cooking oil
has effect
on survival of mosquito larvae
Then
Citrus aurantium juice+peel
Cymbopgon+H2O+Alcohol
Palm-based cooking oil
will kill
mosquito larvae
Objectives
Discover if
Citrus aurantium juice+peel
Cymbopgon+H2O+Alcohol
Palm-based cooking oil
can be
used as larvicide
(reduce chance of survival of mosquitoes)
Scope and Limitations
research conducted to find out
which among:
Citrus aurantium juice+peel
Cymbopgon+H2O+Alcohol
Palm-based cooking oil
can control population
of mosquitoes by killing its larvae
Only
mosquito larvae
one source of larvae
October to December
behavior of larvae
no. of adult mosquitoes present
for contained bodies of water
Methods
Materials
30 mL of palm-based cooking oil, 30 mL of Cymbopogon essential oil, water and ethyl alcohol solution and 30 mL of a mixture of the ground peel and juice of a Citrus aurantium will serve as the treatments
50 mL beaker
graduated cylinder
4 labeled plastic cups
water and mosquito larvae
walled tray
marker, paper, scotch tape
sink and dishwashing liquid
protective mesh
pesticides
Procedure
Find source of larvae
Gather larvae
get a cup and dip it in larvae-infested water
Prepare treatments
transfer from orig. container
set to 30 mL
transfer to container
Make protective meshes
stockings cut and tied into sac-like "caps"
Treat the experimental set-ups
Pour each treatment into its respective cup
Secure protective meshes onto cups
using rubber bands
Collect data
For 7 days, the number of adult mosquitoes present will be recorded every 24 hours.
Clean-up
Pack away
Tread adult mosquitoes with pesticide
Dispose dirty water
Results
TABLE 1. Mean of Number Adult Mosquitoes Present in set-ups with different Treatments applied over the course of Ten Trials
FIGURE 1. Bar Graph of Mean of Adult Mosquitoes Present in set-ups with different Treatments applied over the course of Ten Trials
TABLE 2. Qualitative Observations/Remarks on the Experimental Set-ups
Discussions
Oils
Proven to kill mosquito larvae
larvae suffocates
Cymbopogon essential oil
can kill mosquito larvae
Alcohol
major component of KAAD solution
can kill mosquito larvae
Citrus aurantium mixture
too old
not acidic enough
Conclusions
Hypothesis partially accepted
The solution of ethyl alcohol, water, and essential oil of Cymbopogon, and palm-based cooking oil affected the larvae in their respective set-ups as hypothesized
However, the Citrus aurantium mixture did not affect the larvae as hypothesized
Investigatory Project 2014
By Julian Patdu
