CBIOMES Annual Meeting (2021)
Jesse McNichol, Postdoc, Fuhrman Lab
University of Southern California
Progress & prospects for using universal primers in modelling work
Single assay for Bacteria, Archaea & Eukarya
Microbe art: @claudia_traboni
"ASVs" = (Exact) Amplicon Sequence Variants
A comprehensive picture of phytoplankton
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
What do relative abundances mean?
Scaled by total chlorophyll (CTD)
An integrative framework
+ Chlorophyll
+ Flow cytometry
Scaled ASV data
r_i^{j} = t_i a^{j} Q_i n_{i}^{j}
Mapping model traits to ASV data
ASV DNA data
ASV phylogeny
Using the full dataset
Worden et al., 2015 (Science)
Full community profile
Single cell genomes from Pachiadaki et al., 2019
Network inference
Future collaboration
pmel.noaa.gov/eoi/staff/resing/clivar.html
Reygondeau et al., 2013
Conclusions
1. New datasets
2. DARWIN - data comparisons
3. Data for collaboration
4. Expanding reach of models
Acknowledgements
Paul Berube & Steven Biller
Yi-Chun Yeh (PhD candidate, Fuhrman lab)
Questions?
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
Model-data intercomparison (GA03)
CBIOMES2021_eASVs
By jcmcnch
