Figure 1: Overview of sampling and data processing workflow. Oxygen and sulfide depth profiles shown in blue and orange, respectively. SRF=surface, RXL=redoxcline, EUX=euxinic. 3DMB=3-domain metabarcoding, MAGs = metagenome assembled genomes, FL-16S = full-length 16S. OOI = organism of interest. FISH = fluorescence in-situ hybridzation.

[H2S]

[O2]

depth

EUX ~3°C

RXL ~10°C

SRF ~22°C

DNA

-3DMB

-MAGs

-FL-16S

OOI 

 

(function,

microdiversity)

(broad group level,

microdiverse subcluster level)

FISH probes

 

OOI-specific measurements:

-Growth rate

-Respiration

-Resilience

Field sampling

Lab processing

Field incubations

NSERC overview figure

By jcmcnch

NSERC overview figure

Overview figure for 2025 NSERC application

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