Feng Ling PRO
Postdoc @ Helmholtz Pioneer Campus, studying biophysics, cilia, and mucus flow. Looking for Universality from Diversity.
Quantitative Prediction of Ciliated Organ Function through Unified Fluid Modeling
凌 峰
(Feng Ling)
第二十一届生物物理大会 - 微流控细胞分析
亥姆霍兹 慕尼黑 研究所
Helmholtz Pioneer Campus / IBMI / TranslaTUM
(运动纤毛器官流体泵送功能的量化分析与预测)
内容梗概
‣ 真核细胞纤毛的生物物理与人类健康
‣ 跨尺度动力学分析中的难题与瓶颈
‣ 器官层面纤毛流体泵送的量化分析
‣ 人体纤毛组织的高通量微流控表型
/ Contents
真核细胞纤毛的生物物理与人类健康
Motile Micro-filaments: Flagella, Archella, and Cilia
Cammann, et al, arXiv (2025)
Beeby et al, FEMS Microbiology Reviews (2020)
adapted from Kirsty Y. Wan, Essays Biochem. (2018)
volvox colony
choanoflagellate
rosetta
sperm
stentor
worm larva
frog embryo
mouse brain
ventricles
human
lung
bi-flagellate
quadri-
flagellate
Almost every single Eukaryotic cell possesses at least one type of cilium.
Motile Cilia often serve critical fluid mechanical and signaling functions in animals.
length
scale
真核细胞纤毛的生物物理与人类健康
Motile Cilia - Ubiquitous for Eukaryotic Movements
motile cilia
primary cilia
Mill et al., Nature Review Genetics (2023)
Lin, Nicastro, Science (2018)
Dynein
microtubule
doublets
Cilia motility are enabled by highly conserved axoneme substructure : dyneins on MT doublets.
Walton, 桂淼, et al., Alan Brown, Nature (2023)
Highly Conserved Power Generating Units
Exact force regulation mechanisms and inter-cilium coordination dynamics is still NOT fully resolved!
waveform bistability
真核细胞纤毛的生物物理与人类健康
d
Figuring out how cilia move could seriously demystify cellular motility, mechanics in single-to-multicellular transition, and MANY human diseases!
Understanding Cilia for Biophysics & Human Disease
四纤毛藻
Wan K.Y., Phil Trans B (2019)
30μm
Hamel, et al. (2010)
草履虫
30μm
© Frank Fox (2011)
团藻
Ueki, et al. PNAS (2012)
哺乳动物脑室壁
纤毛流场
unpublished © Nawroth Lab (2025)
20μm
Pellicciotta, et al. PNAS (2020)
呼吸道
纤毛清污
Roth, Sahin, Ling et al., Nat Comm (2025)
100μm
30μm
鱿鱼荧光器官
有益细菌过滤
Nawroth, et al., PNAS (2017)
Shekhar, et al., Nat Phys (2025)
喇叭纤虫群
精子
袁军华组, Lab Chip (2024)
© Journey to the Microcosmos (2019)
眼虫/裸藻
30μm
四纤毛藻
Wan K.Y., Phil Trans B (2019)
30μm
Hamel, et al. (2010)
草履虫
30μm
© Frank Fox (2011)
团藻
Ueki, et al. PNAS (2012)
哺乳动物脑室壁
纤毛流场
unpublished © Nawroth Lab (2025)
20μm
Pellicciotta, et al. PNAS (2020)
呼吸道
纤毛清污
Roth, Sahin, Ling et al., Nat Comm (2025)
100μm
30μm
鱿鱼荧光器官
有益细菌过滤
Nawroth, et al., PNAS (2017)
Shekhar, et al., Nat Phys (2025)
喇叭纤虫群
精子
袁军华组, Lab Chip (2024)
© Journey to the Microcosmos (2019)
眼虫/裸藻
30μm
真核细胞纤毛的生物物理与人类健康
dynein -> cilia oscillation
multiciliated cell and tissue
ciliated epithelial barrier in organs
cerebral signal transduction
lung disease and infections
gamete transport
length
scale
nanometer
micrometer
milimeter
d
d
d
'conserved' structures ->
diverse oscillation types
hydrodynamic coupling ->
cilia phase coordination
cilia activity distribution ->
pumping and clearance functions
⠀
⠀
⠀
Kanale*, Ling*, et al., PNAS (2022)
Ling, et al., Nature Physics (2024)
Roth*, Sahin*, Ling, et al., Nat. Commun. (2025)
Ling, et al., J. R. S. Interface (2018)
Man*, Ling*, et al., Phil. Trans. B (2020)
跨尺度动力学分析中的难题与瓶颈
MICROSCOPIC
MESOSCOPIC
MACROSCOPIC
3D instability
waveform bistability
in vitro human primary cell
muco-ciliary
clearance
Unresolved : Multiscale Structure-function Maps
d
Flow physics guides morphology of ciliated organ
Ling, et al., Nature Physics (2024)
In Pressure-flowrate space, ciliated organ from 26 phyla collapses right on top of the most efficient curves!
Most Efficient Pumping
'ciliary carpet'
d
d
d
d
d
'ciliary flame'
d
d
d
d
d
Inefficient Design 1
Inefficient Design 2
\(\kappa/\mu=1\) [μm\(^{-2}\)]
器官层面纤毛流体泵送的量化分析
Eva Kanso
Janna Nawroth
In Pressure-flowrate space, ciliated organ from 26 phyla collapses right on top of the most efficient curves!
⠀
Quantitative input-output standards for motile cilia
Structural analysis for underperforming conditions
Proves that human trachea epithelial fundamentally have different planar ciliary structure and clearance capabilities than rat and mouse models!
Proves that human trachea epithelial fundamentally have different planar ciliary structure and clearance capabilities than rat and mouse models!
Roth*, Sahin*, Ling, et al., Nat. Commun. (2025)
Janna Nawroth
Ayşe Tuğçe Şahin
Doris Roth
器官层面纤毛流体泵送的量化分析
Quantitative metric for ex-vivo / in-vitro models
Where's microfluidics? Am I in the wrong session?
人体纤毛组织的高通量微流控表型
Those are in fact necessary ground work before we carry out the painful fun labor-intensive work
(in a way we prefer) because...
- Need to show fluid mechanics are universal factors AND give reproducible quantitative metrics;
- Want to culture and analyze as many cells as close to in-situ as possible with best optical clarity;
- Want live high-speed footage at the same time as time lapse tracking of PRIMARY cell cultures;
- Want to resolve multiscale details and get physically interpretable features ... ...
Possible Solutions:
- pure algorithmic approach: end-to-end deep learning (still at nematode level w/o HQ data/model\(^*\))
- bio-engineering tissues: inverted culture chips\(^1\), folding membrane\(^2\) ... ...
\(*\):
Midtvedt, et al., Nat. Commun. (2022)
Pineda, et al., Nat. Mach. Intell. (2023)
Alonso, et al., Commun. Biol. (2023)
Weheliye, et al, bioRxiv (2024)
Yu, et al., Sci. Rep. (2025)
Deserno, et al, bioRxiv (2025)
\(1\): Kurmashev, et al, Hierlemann, Adv. Mater. Technol. (2024)
\(2\): Causa, Das, et al., Cicuta, PNAS (2025)
人体纤毛组织的高通量微流控表型
High-throughput single-cell mechanical analysis
In Vitro
Patient-Derived
Primary Cell
Cultures
Text
Microfluidics- Trapped
Dissociated
in vitro
Ciliated Cell
Text
sync.
dexioplectic
'antiplectic'
'symplectic'
low beat freq.
low density
incoherent polarity
low amplitude
2025-07-07 © Feng Ling, Nawroth Lab, Omran Lab
PRELIMINARY RESULTS / DO NOT USE
2025-07-07 © Feng Ling, Nawroth Lab, Omran Lab
2025-07-07 © Feng Ling, Nawroth Lab, Omran Lab
10 μm
simulation
simulation
广告:跨尺度生物力学建模与高通量表型
光学仿真
合成数据
⠀
跨尺度
力学建模
⠀
体外模型
高速显微
⠀
纤毛姿态
反演计算
微观动力
学参数
分子马达
超分辨成像
⠀
光学仿真
合成数据
⠀
跨尺度
物理模型
⠀
纤毛姿态
反演计算
细胞黏液微流变测量
鱼群运动降阶模拟
⠀
年底将加入南开大学物理科学院主攻:
⠀
还将开展以下理论、计算为主研究:
Funding Agencies
Collaborators & Acknowledgements
ERC StG 950219
PoC 101212905
NIH R01
HL152633
NSF 160844
ARO W911NF-16-1-0074
ONR N00014-17-1-2062
Thanks for staying to the end!
bye-bye!
欢迎与各位专家交流合作
Yohannes Tesfaigzi
(Harvard Medical School)
Sylvester Holt / Vandana Kaushal
(University of Copenhagen)
Heymut Omran / Sebastian George
(Muenster University Hospital)
Ruth Olmar / Jaboreck Mark-Christian
(Hannover Medical School)
满怡 (北京大学)
郭涵亮 (Ohio Wesleyan University)
Michael J. Shelley
David Stein
(Flatiron / NYU Courant)
Sebastian Fuerthauer
(TU Wien)
Amy L. Ryan
(University of Iowa)
Kakani Katija (MBARI)
Margaret McFall-Ngai
(Carnegie/Caltech)
By Feng Ling