client and cloud computing for 3D reconstruction
and analysis of biomarkers
in sectioned tissue for
pathology or histology
 

contact info
insert here

histo.io

why are we here?

contents

a.    Pitch Deck:   product, buisiness model, vision, team.

b.    Intellectual Asset Portfolio: what is unique about the product?

c.    Market: size, growth, beachhead, dynamics, competition.

d.    Monetization: B2C and B2B offers.

e.    Operation: costs, legal compliance, headcounts.

f.     Appendix: appendix

pitch deck

• cost of drug discovery has skyrocketed.
• despite advances in high-throughput biotechnology (cloning, sequencing)
• high-throughput still requires manual work by highly qualified personnel.
  Especially the part of data analysis and bioinformatics.

parts of the R&D process has improved yet overall efficiency has declined

Eroom's law

Shannell et al. (2012) Nature. Rev. Drug Discovery

pitch deck

• cost of drug discovery has skyrocketed.
• despite advances in high-throughput biotechnology (cloning, sequencing)
• high-throughput still requires manual work by highly qualified personnel.
  Especially the part of data analysis and bioinformatics.

parts of the R&D process has improved yet overall efficiency has declined

Eroom's law

Shannell et al. (2012) Nature. Rev. Drug Discovery

pitch deck

meanwhile sequencing costs have plummeted

genome.gov/sequencingcosts

histology meets nextgen sequencing

pitch deck

arrayed oligos

TomoSeq

in situ sequencing

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Principle: Capturing mRNA
on an arrayed microscope slide

Principle:
Mechanical sectioning
subsequent reconstruction

Principle:
Reverse transcription and amplification directly in tissue. 

The product is a better way to find biomarkers for researchers

pitch deck

labeling of biomarkers in tissue

in situ hybdridization (ISH)

immmunohistochemistry (IHC)

fluorescent proteins (FP)

signal to noise ratio

• Target: RNA / DNA
• Probe: cDNA/cRNA

• Target: Endogenously expressed proteins
• Probe: antibodies

• Target: Transgenically expressed proteins
• Probe: viral vectors, transgenic animals.

The product is a better way to find biomarkers for researchers

pitch deck

tissue processing in research and pathology

• Tissue handling, sectioning, labeling and imaging has largely been automized for routine tasks.
   
• Analysis and exploration of result is largely done by manual inspection.

The product is a better way to find biomarkers for researchers

pitch deck

Manual Image capturing of tissue

sections

Manual transfering and stitching of image tiles

Manual Post Processing in 3rd party software (photoshop, ImageJ)

Manual extraction and annotation
of labeled features

Manual image compression

Manual statistical analysis

Manual visualization and sharing of findings

today's workflow for tissue analysis for biomarker discovery

Manual catalogization of images

Manual identification of reagent

Manual validation of reagent inhouse

Biomarker discovery

Manual ordering of reagent from 3rd party

The product is a better way to find biomarkers for researchers

pitch deck

Manual Image capturing of tissue

sections

WholeBrain
desktop or server client

WholeBrain
cloud storage and
analysis

workflow with wholebrain

Biomarker discovery

automated processing of terabyte of
image data

collaborative platform in the cloud

openbrainmap.org

The product is a better way to find biomarkers for researchers

pitch deck

MUSD/Biomarker

days/Biomarker

125% cheaper

100x faster

today's workflow

with WholeBrain

business model

pitch deck

4-sided. B2B and B2C clients

"Ad-words" like model where Reagents suppl. pay for reagent impressions next to relevant user data that match their products

Exclusive Rev-share/licensing model for differentiation vs other Microscopy vendors

WholeBrain
 

Academic Researcher

• universities
• institutes

Microscopy manufactures

• Zeiss
• Leica
• Olympus
• Nikon

Rev-share

Reagents suppliers

• Sigma-Aldrich
• Thermo-Fisher
• Jackson ImmunoResearch
• Synaptic systems

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SDK

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Transaction and Data
Basic software + Ads
 

client
prioritization

Industry Researcher

• biomarker drug discovery
  Astra Zeneca

• Subscription based business model to cater for ownership of data and company specific requirements.

Preemium
license

• Freemium model with transactional based  add-ons.

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Subscription fee
 

Money for data/ads
Basic User data or
ad-impressions
 

the team

pitch deck

daniel fürth

viktor ängmo

professional experience

• Business Development Manager
  - MTG technology
• Part-owner and business development
  - Luxbright (cathode x-ray tubes)
   

• Education
  MSc/MMed in Entreprenuership & Business Creation Biotechnology
  - Chalmers University of Technology & Gothenburg University

professional experience

• Doctoral student
  - Department of Neuroscience,         Karolinska Institutet

Education

• Bachelor in Medical Science
  - Karolinska Institutet

contents

a.    Pitch Deck:   product, buisiness model, vision, team.

b.    Intellectual Asset Portfolio: what is unique about the product?

c.    Market: size, growth, beachhead, dynamics, competition.

d.    Monetization: B2C and B2B offers.

e.    Operation: costs, legal compliance, headcounts.

f.     Appendix: appendix

software suite

intellectual asset portfolio

openbrainmap.org

WholeBrain

open brain map

fisseq with R

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Technology:
deskop application for image processing of microscope images of tissue

Technology:
'Google Maps'-like cloud based interface for neuroanatomical data. Since the brain has a particular anatomy not seen in any other organ.

Technology:
desktop application for image enhancement and basecalling, sequence alignment and bioinformatics in high-dimensional big data (> 1Tb)  next-gen sequencing raw FISSEQ images. 

cloud storage

intellectual asset portfolio

front end
user verification
account

cloud SSD backend

AWS S3 storage

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• End user registers for an account at histoflurp.com Freemium model up to a certain limit (10 Gb).
   
• histoflurp.com account is linked against our cloud backend which with SSD can quickly serve up images and content for use with our software suite.
   
• Permanent storage of images is done at
  amazon S3 AWS.

researcher
end user

contents

a.    Pitch Deck:   product, buisiness model, vision, team.

b.    Intellectual Asset Portfolio: what is unique about the product?

c.    Market: size, growth, beachhead, dynamics, competition.

d.    Monetization: B2C and B2B offers.

e.    Operation: costs, legal compliance, headcounts.

f.     Appendix: appendix

Next-Gen Sequencing for oncology biopsy is our beachhead market

market

• The primary industry of focus is the industry for Wholebrain is in the next gen sequencing market for Oncology.  
   
• Current therapy selection decisions are based on the outcome of a biopsy tests. Most common tests are immunoassays, sequencing or Histology to select a treatment.
   
• The ideal test can provide the pathologist with enough data to prescribe the correct therapy and not risk of prescribing a therapy without effect.
   
• The current state of data granularity on the sequencing methods is “single-cell – gene expression”. It can reveal rare cellular properties and biologically meaningful cell-to-cell variability. Still,  pathologists are blindfolded when it comes to understanding the cause of the cancer for a patient in subject. Resulting in risk of wrong therapy selection.  
   
• To be able to reach the ideal test, two pieces of data needs to be added to the biopsy test. 1) sub-cell building blocks (mRNA) and 2) spatial information. With this information you are able to find the driver of mutations and the pathways.
   
• The oncology sequencing market now embarks on a next phase, where numerous of companies all across the value chain will collectively work towards the goal of reaching this “Spatially-resolved Transcriptomics”.

Oncology Sequencing Market State

Sequencing is one method for choosing oncology therapy

Oncology
Health care chain

Predict

Diagnose

Inform

Treat

Risk assessment

Definitive diagnosis

Prognosis

Screening

Therapy selection

Development
resistance screening

Monitoring

Follow-up

Diagnosis

Histopathology

Immunoassays

Sequencing

NextGen Sequencing (NGS) market is estimated to grow at  20% CAGR and reach 12 billion USD in 2021

market

​Our main target group are Research Institutes buying, reagents in North America and Europe working on RNA based methods as they make up the vast majority of the market.

Total NGS Market 2016-2021 (BUSD)

Total NGS Market segmented (2015)

Market entering strategy

market

We can enter that market by grabbing a share of the Tertiary Software Market that is suffering from poor tools to provide insights on what reagents to buy

Annual budget for software to analyze NGS data

Average time spent analyzing NGS data

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The market is fueled by massive investments in precision based medicine

market

our vision

pitch deck

a long lasting impact on spatial information handling and analysis in pathology, diagnostics and microscopic image analysis

• Essentially doing for pathology and microscopic image analysis what Google did for geography.
   
• The field of microscopic image analysis is akin to cartography in a pre Google Maps era.
   
• Both data collection and analysis is still largely manual.
   
• There is no central source where data can be anchored in a spatial reference frame.

every biological process takes place somewhere