Basin-scale biogeography with 3-domain ASVs
What is the resolution of ASVs?
- Are Pro and Syn indistinguishable?
- Prochlorococcus ecotypes distinguishable?
What is the resolution of ASVs?
- Are Pro and Syn indistinguishable?
- Yes, sometimes, but rarely
- Prochlorococcus ecotypes distinguishable?
- Yes, for most ASVs
What is the resolution of ASVs?
- New databases:
- SAR11 ecotypes
- Phytoplankton 16S / 18S
- Link traits such as size to ASVs?
ASV phylogeny
P16N/S
16S phytoplankton (EUK vs PROK)
Samples courtesy of Craig Carlson
P16N/S
16S phytoplankton
Samples courtesy of Craig Carlson
P16N/S
16S minus cyanobacteria + mitochndria
Samples courtesy of Craig Carlson
P16N/S bacterioplankton
P16N/S bacterioplankton
P16N/S bacterioplankton
P16N/S bacterioplankton
P16N/S bacterioplankton
P16N/S bacterioplankton
Next steps: Network analysis
plastid
16S
mito 16S
nuclear 18S
Space
Abundance
A eukaryotic phytoplankter
(Artist's impression, not real data)
Next steps: CMAP
- Assist the Armbrust group ingesting GEOTRACES chemical data into CMAP
- Manual curation required - Yubin and I are working with Tansy, Diana and Rhonda
- ASV data to CMAP
- GA03
- GP13
- GA02
- GA10
- P16N-S
- I8/I9
- AMT
- nifH qPCR vs amplicons
OSM / CBIOMES materials
By jcmcnch
