Acknowledgements (much more later)
Committee
Dr. Jacob Robinson
Dr. Robert Raphael
Dr. Daoyun Ji
Lab
Dr. Caleb Kemere
Josh, Ziying, Sibo, Della, Kayla, Dani, Amy
Former: Jim, Ariel, Etienne, Alex, James, Jill, Eric
Interrogating the role of hippocampal sharp-wave ripples in spontaneous learning
Shay(ok) Dutta
April 25, 2023
Dr. Caleb Kemere (Chair)
Dr. Jacob Robinson
Dr. Robert Raphael
Dr. Daoyun Ji
Where to begin?
Active Behavior
Quiescence
hmm...
Where to begin?
Quiescence
hmm...
Active Behavior
What does this look like?
What does this look like?
What does this look like?
Where to begin?
Quiescence
hmm...
Active Behavior
How does the brain store this configuration of the world?
Background
Knerim et al. Philosophical Transactions of the Royal Society B: Biological Sciences 2014
Clark et al. J. Neuro 2000 showed lesioning HPC impairs novelty preference
Hippo 101
Hippo 101
Hippo 101
Are SWRs related to storing the configuration of the world?
SWRs & ORM
Larkin et al. Hippocampus 2014
Are SWRs related to storing the configuration of the world?
How can we test this?
Dutta et al. JNE 2018
Let's see it in action!
Are we doing it right?
Dutta et al. JNE 2018
Are we doing it right?
Dutta et al. JNE 2018
Let's check out validity!
Hippo 101 SWRs
Hippo 101 SWRs
Hippo 101
SWR
- Eventually the animal learns the task
- Not repeatable for an extended amount of time
- Food restricted and goal directed not spontaneous and innate curiosity
Let's test this!
ORM No More!
Are SWRs related to storing the configuration of the world?
YES!
Hippo 101
SWR
So, why is this cool again?
- Ripples have been causally linked to object-location memory consolidation!
- Natural, foraging, spontaneous memory task. No reward or forced structure!
- The disruption and chance level discrimination between objects is a result that can be achieved day-after-day
Object Recognition Memory
Object Recognition Memory
Object Recognition Memory
What is the disruption doing?
What is the disruption doing?
What is the disruption doing?
What is the disruption doing?
Trying to target long ripples
Trying to target long ripples
Trying to target long ripples
In action!
Long ripple disruption!
Conclusions
Contributions of this thesis
- Causal relationship established between hippocampus and object-location memory
- First ripple disruption task that can be run repeatedly with chance level performance!
- New memory formation, consolidation and place field stability questions can be asked!
- First ripple disruption task with result with a behavioral effect that is in shown in a natural, foraging, curiosity driven behavior innate across animals!
- Longer ripples clearly have some relationship with better memory consolidation but all ripples are matter for consolidation!
Real Conclusion
- September 2014: I asked Caleb what a life cycle of a PhD Student in his lab was based on his vision
- August 2015: I asked Jacob what a life cycle of a PhD Student in his lab was based on his vision.
Questions?
Acknowledgements
Committee
Dr. Jacob Robinson
Dr. Robert Raphael
Dr. Daoyun Ji
Lab
Dr. Caleb Kemere
Josh, Ziying, Sibo, Della, Kayla, Dani, Amy
Former: Jim, Ariel, Etienne, Alex, James, Jill, Eric
Vivarium staff!
Kelly
Elysse (first name :P!)
(I'm going to miss some other folks if I start listing out names!)
So many other Rice folks
Joe, Jon, probably more J names, maybe Kelly?, etc.
Acknowledgements
BRC Security
Acknowledgements
Al Vetro (former) Amilles Coffee (current)
Acknowledgements
Caleb.....
Acknowledgements
OffendingAPhD
By Shayok Dutta
OffendingAPhD
This presentation explores the role of hippocampal sharp-wave ripples in spontaneous learning, with a particular focus on object recognition memory.
