Interrogating the role of hippocampal sharp-wave ripples in spontaneous learning
Shay(ok) Dutta
June 14, 2023
Acknowledgements
Monies!
NSF CAREER (CBET-1351692), NSF BRAIN
EAGER (IOS-1550994), NIH R01NS115233, HFSP Young Investigator’s (RGY0088)
Lab
Current: Dr. Caleb Kemere
Ziying, Sibo, Della, Kayla, Dani
Former: Drs. Josh, James, Etienne, Alex; Mentees! Amy (MD stud!), Jim (NSF GRFP!), Ariel (Carnegie Student Fellow!); MS! Jill, Eric
Others
Drs. Jacob Robinson, Rob Raphael, Jon Flynn, Joe Young, Daoyun Ji, Amanda Singer, Elysse Orchard, MS! Kelly Kim/Campbell
💰
Interrogating the role of hippocampal sharp-wave ripples in spontaneous learning
Where to begin?
Active Behavior
Quiescence
hmm...
Where to begin?
Quiescence
hmm...
Active Behavior
What does this look like?
What does this look like?
What does this look like?
Where to begin?
Quiescence
hmm...
Active Behavior
How does the brain store this configuration of the world?
Background
Knerim et al. Philosophical Transactions of the Royal Society B: Biological Sciences 2014
Clark et al. J. Neuro 2000 showed lesioning HPC impairs novelty preference
Hippo 101
Hippo 101
Figure concept adapted from Colgin, Nat. Neuro. Reviews 2015
Hippo 101
Are SWRs related to storing the configuration of the world?
Figure concept adapted from Colgin, Nat. Neuro. Reviews 2015
SWRs & ORM
Larkin et al. Hippocampus 2014
Are SWRs related to storing the configuration of the world?
How can we test this?
Dutta et al. JNE 2018
Let's see it in action!
Are we doing it right?
Dutta et al. JNE 2018
Are we doing it right?
Dutta et al. JNE 2018
Let's check out validity!
Let's test this!
ORM No More!
Are SWRs related to storing the configuration of the world?
YES!
Hippo 101
SWR
So, why is this cool again?
- Ripples have been causally linked to object-location memory consolidation!
- Natural, foraging, spontaneous memory task. No reward or forced structure!
- The disruption and chance level discrimination between objects is a result that can be achieved day-after-day
Object Recognition Memory
Object Recognition Memory
Object Recognition Memory
What is the disruption doing?
What is the disruption doing?
Is this a reasonable or fair comparison across animals?
What is the disruption doing?
Is this a reasonable or fair comparison across animals?
What is the disruption doing?
There seems to be a difference in the distributions!?
What is the disruption doing?
Trying to target long ripples
Trying to target long ripples
How can we modify this algorithm to detect longer ripples?!?!
Trying to target long ripples
In action!
Long ripple disruption!
Conclusions
Contributions (i.e., I've scienced!?)
- Causal relationship established between hippocampus and object-location memory
- First ripple disruption task that can be run repeatedly with chance level performance!
- New memory formation, consolidation and place field stability questions can be asked!
- First ripple disruption task with result with a behavioral effect that is in shown in a natural, foraging, curiosity driven behavior innate across animals!
- Longer ripples clearly have some relationship with better memory consolidation. Perhaps they are needed for full strtength of memory?
Real Conclusion
- September 2014: I asked Caleb what a life cycle of a PhD Student in his lab was based on his vision
- August 2015: I asked Jacob what a life cycle of a PhD Student in his lab was based on his vision.
Questions?
What I hope to do here!
Fear, anxiety, depression, stress, etc. & therapeutic modulation?
(i.e., combining things --> actually contribute to humans!?)
Zheng & Colgin Nat. Neuro. News & Views 2017
Hippo Background
Wu et. al, Nat. Neuro. 2017
Zheng & Colgin Nat. Neuro. News & Views 2017
Proposal for Cool Things?
SWR-ORM-Fear-Talk
By Shayok Dutta
SWR-ORM-Fear-Talk
This presentation explores the role of hippocampal sharp-wave ripples in spontaneous learning, with a particular focus on object recognition memory.
